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Product Info Summary
| SKU: | PA1591 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Histone deacetylase 8 HDAC8 Antibody Picoband®
View all Histone Deacetylase 8/HDAC8 Antibodies
SKU/Catalog Number
PA1591
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Histone deacetylase 8 HDAC8 Antibody catalog # PA1591. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone deacetylase 8 HDAC8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1591)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HDAC8, different from the related mouse and rat sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1591 is reactive to HDAC8 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
41758 MW
Background of Histone Deacetylase 8/HDAC8
HDAC8, Histone deacetylase 8, is an enzyme that in humans is encoded by the HDAC8 gene, and is biologically involved in skull morphogenesis and metabolic control of the ERR-alpha/PGC1-alpha transcriptional complex. Sequence analysis predicted that the 377-amino acid HDAC8 protein contains the 9 conserved HDAC blocks that are presumably important for catalytic function. HDAC8 shares 54% sequence similarity with HDAC1 and HDAC2 and 39% similarity with HDAC3, making it a class I HDAC. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1591 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Cardiac Muscle: Tissue, A549 Whole Cell
IHC: Human Lung Cancer Tissue
Validation Images & Assay Conditions
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Anti-HDAC8 antibody, PA1591, Western blotting
All lanes: Anti HDAC8 (PA1591) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle: Tissue Lysate at 50ug
Lane 2: A549 Whole Cell Lysate at 40ug
Predicted bind size: 42KD
Observed bind size: 42KD
Click image to see more details
Anti-HDAC8 antibody, PA1591, IHC(P)
IHC(P): Human Lung Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For HDAC8 (Source: Uniprot.org, NCBI)
Gene Name
HDAC8
Full Name
Histone deacetylase 8
Weight
41758 MW
Superfamily
histone deacetylase family
Alternative Names
Histone deacetylase 8;HD8;3.5.1.98;HDAC8;HDACL1;CDA07; HDAC8 CDA07, CDLS5, HD8, HDACL1, KDAC8, MRXS6, RPD3, WTS histone deacetylase 8 histone deacetylase 8|histone deacetylase-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HDAC8, check out the HDAC8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HDAC8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histone deacetylase 8 HDAC8 Antibody Picoband® (PA1591)
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Customer Q&As
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2 Customer Q&As for Anti-Histone deacetylase 8 HDAC8 Antibody Picoband®
Question
I am interested in your PA1591 HDAC8 antibody. Since many reference showed HDAC8 majorly located at nucleus, however, your data seems located at cytoplasm. So may I know if you have other data with clear location signal to know if the signal stained by this antibody located at nucleus or cytoplasma?https://www.spandidos-publications.com/10.3892/or.2016.5280 Thank you
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
The signal stained by our lab technicians using PA1591 is located at
Boster Scientific Support
Answered: 2019-06-17
Question
We are currently using anti-HDAC8 antibody PA1591 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-05
Answer
The anti-HDAC8 antibody (PA1591) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-05
