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Product Info Summary
| SKU: | M01843-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal Antibody
View all Histone Deacetylase 8/HDAC8 Antibodies
SKU/Catalog Number
M01843-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal Antibody catalog # M01843-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01843-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HGO-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HDAC8
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01843-1 is reactive to HDAC8 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated molecular weight
41758 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01843-1 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:20000
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: HeLa cell
ICC/IF: HELA cell
Validation Images & Assay Conditions
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Western blot analysis of HDAC8 expression in HeLa cell lysate (M01843-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC8 monoclonal antibody (Catalog # M01843-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC8
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IF analysis of HDAC8 using anti-HDAC8 antibody (M01843-1) and anti-Beta Tubulin antibody (M01857-3).
HDAC8 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 with rabbit anti-HDAC8 Antibody (M01843-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For HDAC8 (Source: Uniprot.org, NCBI)
Gene Name
HDAC8
Full Name
Histone deacetylase 8
Weight
41758 MW
Superfamily
histone deacetylase family
Alternative Names
Histone deacetylase 8;HD8;3.5.1.98;HDAC8;HDACL1;CDA07; HDAC8 CDA07, CDLS5, HD8, HDACL1, KDAC8, MRXS6, RPD3, WTS histone deacetylase 8 histone deacetylase 8|histone deacetylase-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HDAC8, check out the HDAC8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HDAC8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal Antibody (M01843-1)
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Customer Q&As
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6 Customer Q&As for Anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal Antibody
Question
Is this M01843-1 anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal antibody reactive to the isotypes of HDAC8?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
The immunogen of M01843-1 anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal antibody is A synthesized peptide derived from human HDAC8 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-09
Question
I was wanting to use your anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal antibody for WB for rat colon small intestine on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat colon small intestine identification?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
As indicated on the product datasheet, M01843-1 anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat colon small intestine in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-12
Question
Do you have a BSA free version of anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal antibody M01843-1 available?
T. Anderson
Verified customer
Asked: 2019-05-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal antibody M01843-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-31
Question
Can you help my question with product M01843-1, anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Mitchell
Verified customer
Asked: 2018-10-22
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01843-1 anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-22
Question
I see that the anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal antibody M01843-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-10-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-10-04
Question
Is a blocking peptide available for product anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal antibody (M01843-1)?
O. Johnson
Verified customer
Asked: 2016-09-23
Answer
We do provide the blocking peptide for product anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal antibody (M01843-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-09-23
