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Product Info Summary
| SKU: | M00739 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-HCG-alpha (Pregnancy & Choriocarcinoma Marker) Monoclonal Antibody
SKU/Catalog Number
M00739
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-HCG-alpha (Pregnancy & Choriocarcinoma Marker) Monoclonal Antibody (Catalog # M00739). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-HCG-alpha (Pregnancy & Choriocarcinoma Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00739)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM552
Isotype
IgG1, kappa
Immunogen
Recombinant hCG alpha protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M00739 is reactive to CGA in Human
Reconstitution
Reconstitute with distilled water.
Calculated molecular weight
13075 MW
Background of CGA
This monoclonal antibody reacts with a protein of ~13kDa, identified as alpha sub-unit of HCG. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the subunit is identical to that of thyroid stimulating hormone (TSH) follicle stimulating hormone (FSH), and luteinizing hormone (LH).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00739 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Placenta stained with Anti-hCG alpha Monoclonal Antibody (SPM552).
Protein Target Info & Infographic
Gene/Protein Information For CGA (Source: Uniprot.org, NCBI)
Gene Name
CGA
Full Name
Glycoprotein hormones alpha chain
Weight
13075 MW
Superfamily
glycoprotein hormones subunit alpha family
Alternative Names
Glycoprotein hormones alpha chain;Anterior pituitary glycoprotein hormones common subunit alpha;Choriogonadotropin alpha chain;Chorionic gonadotrophin subunit alpha;CG-alpha;Follicle-stimulating hormone alpha chain;FSH-alpha;Follitropin alpha chain;Luteinizing hormone alpha chain;LSH-alpha;Lutropin alpha chain;Thyroid-stimulating hormone alpha chain;TSH-alpha;Thyrotropin alpha chain;CGA; CGA CG-ALPHA, FSHA, GPA1, GPHA1, GPHa, HCG, LHA, TSHA glycoprotein hormones, alpha polypeptide glycoprotein hormones alpha chain|FSH-alpha|LSH-alpha|TSH-alpha|anterior pituitary glycoprotein hormones common subunit alpha|choriogonadotropin alpha chain|chorionic gonadotrophin subunit alpha|chorionic gonadotropin, alpha polypeptide|follicle-stimulating hormone alpha chain|follicle-stimulating hormone alpha subunit|follitropin alpha chain|luteinizing hormone alpha chain|lutropin alpha chain|thyroid-stimulating hormone alpha chain|thyrotropin alpha chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CGA, check out the CGA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CGA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HCG-alpha (Pregnancy & Choriocarcinoma Marker) Monoclonal Antibody (M00739)
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