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Product Info Summary
| SKU: | M30942 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-LH, alpha (Luteinizing Hormone, alpha) CGA Monoclonal Antibody
SKU/Catalog Number
M30942
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-LH, alpha (Luteinizing Hormone, alpha) CGA Monoclonal Antibody (Catalog # M30942). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-LH, alpha (Luteinizing Hormone, alpha) CGA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30942)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: LHa/756
Isotype
IgG1, kappa
Immunogen
Recombinant full-length hLH alpha protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M30942 is reactive to LHA in Human
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
13075 MW
Background of CGA
This monoclonal antibody reacts with a protein of ~13kDa, identified as alpha sub-unit of Luteinizing Hormone (LH). Its structure is similar to the other glycoproteins, follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH), and human chorionic gonadotropin (hCG). The protein dimer contains 2 polypeptide units, labeled alpha and beta subunits that are connected by two bridges. The alpha subunits of LH, FSH, TSH, and hCG are identical, and contain 92 amino acids. The beta subunits vary. LH has a beta subunit of 121 amino acids (LHB) that confers its specific biologic action and is responsible for interaction with the LH receptor. This beta subunit contains the same amino acids in sequence as the beta subunit of hCG and both stimulate the same receptor; however, the hCG beta subunit contains an additional 24 amino acids and the hormones differ in the composition of their sugar moieties.LH is synthesized and secreted by gonadotrophs in the anterior lobe of the pituitary gland. In concert with the other pituitary gonadotropin follicle-stimulating hormone (FSH), it is necessary for proper reproductive function. In the female, an acute rise of LH levels triggers ovulation. In the male, where LH has also been called Interstitial Cell-Stimulating Hormone (ICSH), it stimulates Leydig cell production of testosterone. LH is a useful marker in classification of pituitary tumors and the study of pituitary disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M30942 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(No special pre-treatment is required for staining of formalin-fixed tissues. Note that HIER is NOT recommended for this antibody.)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Pituitary stained with Anti-LH alpha Mouse Monoclonal Antibody (LHa/756).
Protein Target Info & Infographic
Gene/Protein Information For LHA (Source: Uniprot.org, NCBI)
Gene Name
LHA
Full Name
Glycoprotein hormones alpha chain
Weight
13075 MW
Superfamily
glycoprotein hormones subunit alpha family
Alternative Names
Glycoprotein hormones alpha chain;Anterior pituitary glycoprotein hormones common subunit alpha;Choriogonadotropin alpha chain;Chorionic gonadotrophin subunit alpha;CG-alpha;Follicle-stimulating hormone alpha chain;FSH-alpha;Follitropin alpha chain;Luteinizing hormone alpha chain;LSH-alpha;Lutropin alpha chain;Thyroid-stimulating hormone alpha chain;TSH-alpha;Thyrotropin alpha chain;CGA; CGA CG-ALPHA, FSHA, GPA1, GPHA1, GPHa, HCG, LHA, TSHA glycoprotein hormones, alpha polypeptide glycoprotein hormones alpha chain|FSH-alpha|LSH-alpha|TSH-alpha|anterior pituitary glycoprotein hormones common subunit alpha|choriogonadotropin alpha chain|chorionic gonadotrophin subunit alpha|chorionic gonadotropin, alpha polypeptide|follicle-stimulating hormone alpha chain|follicle-stimulating hormone alpha subunit|follitropin alpha chain|luteinizing hormone alpha chain|lutropin alpha chain|thyroid-stimulating hormone alpha chain|thyrotropin alpha chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LHA, check out the LHA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LHA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LH, alpha (Luteinizing Hormone, alpha) CGA Monoclonal Antibody (M30942)
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Customer Q&As
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6 Customer Q&As for Anti-LH, alpha (Luteinizing Hormone, alpha) CGA Monoclonal Antibody
Question
My question regards to test anti-LH, alpha (Luteinizing Hormone, alpha) Monoclonal antibody M30942 on human placenta for research purposes, then I may be interested in using anti-LH, alpha (Luteinizing Hormone, alpha) Monoclonal antibody M30942 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Banerjee
Verified customer
Asked: 2020-04-21
Answer
The products we sell, including anti-LH, alpha (Luteinizing Hormone, alpha) Monoclonal antibody M30942, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-21
Question
Would anti-LH, alpha (Luteinizing Hormone, alpha) Monoclonal antibody M30942 work for IHC with placenta?
Verified Customer
Verified customer
Asked: 2018-12-06
Answer
According to the expression profile of placenta, CGA is highly expressed in placenta. So, it is likely that anti-LH, alpha (Luteinizing Hormone, alpha) Monoclonal antibody M30942 will work for IHC with placenta.
Boster Scientific Support
Answered: 2018-12-06
Question
We are currently using anti-LH, alpha (Luteinizing Hormone, alpha) Monoclonal antibody M30942 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
S. Anderson
Verified customer
Asked: 2017-12-05
Answer
The anti-LH, alpha (Luteinizing Hormone, alpha) Monoclonal antibody (M30942) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-05
Question
Is a blocking peptide available for product anti-LH, alpha (Luteinizing Hormone, alpha) Monoclonal antibody (M30942)?
Verified Customer
Verified customer
Asked: 2017-07-28
Answer
We do provide the blocking peptide for product anti-LH, alpha (Luteinizing Hormone, alpha) Monoclonal antibody (M30942). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-07-28
Question
My question regarding product M30942, anti-LH, alpha (Luteinizing Hormone, alpha) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-05-16
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M30942 anti-LH, alpha (Luteinizing Hormone, alpha) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-16
Question
Will anti-LH, alpha (Luteinizing Hormone, alpha) Monoclonal antibody M30942 work on feline IHC with pituitary?
Z. Miller
Verified customer
Asked: 2015-09-07
Answer
Our lab technicians have not validated anti-LH, alpha (Luteinizing Hormone, alpha) Monoclonal antibody M30942 on feline. You can run a BLAST between feline and the immunogen sequence of anti-LH, alpha (Luteinizing Hormone, alpha) Monoclonal antibody M30942 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline pituitary in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-09-07
