Click image to see more details
Product Info Summary
| SKU: | A00874-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NEMO/IKK-gamma IKBKG Antibody
SKU/Catalog Number
A00874-1
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-NEMO/IKK-gamma IKBKG Antibody (Catalog # A00874-1). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-NEMO/IKK-gamma IKBKG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00874-1)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-NEMO was affinity purified from whole rabbit serum prepared by repeated immunizations with a recombinant protein of human NEMO.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00874-1 is reactive to IKBKG in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
19891 MW
Background of IKBKG
Anti-NEMO antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Anti-NEMO antibody detects recombinant and endogenous NEMO. NEMO, the regulatory subunit of the IKK core complex, phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. Nemo is also considered to be a mediator for TAX activation of NF-kappa-B and may be implicated in NF-kappa-B-mediated protection from cytokine toxicity. NEMO is essential for viral activation of IRF3 and involved in TLR3- and IFIH1-mediated antiviral innate response. The innate antiviral response from NEMO requires 'Lys-27'-linked polyubiquitination. Anti-NEMO is ideal for researchers interested in Immunology and Cancer research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00874-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:50,000 - 1:400,000
IP: 5µg
WB: 1:500-1:1000
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IKBKG using anti-IKBKG antibody (A00874-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKBKG antigen affinity purified polyclonal antibody (Catalog # A00874-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for IKBKG.
Click image to see more details
Figure 2. Western blot analysis of IKBKG using anti-IKBKG antibody (A00874-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKBKG antigen affinity purified polyclonal antibody (Catalog # A00874-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for IKBKG.
Protein Target Info & Infographic
Gene/Protein Information For IKBKG (Source: Uniprot.org, NCBI)
Gene Name
IKBKG
Full Name
NF-kappa-B essential modulator
Weight
19891 MW
Alternative Names
NEMO, IKBKG, NF-kappa-B essential modulator, FIP3, NEMO, regulatory subunit of the IKK core complex, I-kappa-B kinase subunit gamma, IKK-gamma, IKKG, IkB kinase-associated protein 1, IKKAP1, IKK gamma IKBKG AMCBX1, EDAID1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma NF-kappa-B essential modulator|I-kappa-B kinase subunit gamma|NF-kappa-B essential modifier|ikB kinase subunit gamma|ikB kinase-associated protein 1|incontinentia pigmenti|inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma|inhibitor of nuclear factor kappa B kinase subunit gamma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IKBKG, check out the IKBKG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IKBKG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NEMO/IKK-gamma IKBKG Antibody (A00874-1)
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Customer Q&As
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3 Customer Q&As for Anti-NEMO/IKK-gamma IKBKG Antibody
Question
Is there a BSA free version of anti-NEMO/IKK-gamma antibody A00874-1 available?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NEMO/IKK-gamma antibody A00874-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-01
Question
I would like to test anti-NEMO/IKK-gamma antibody A00874-1 on human lung for research purposes, then I may be interested in using anti-NEMO/IKK-gamma antibody A00874-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Thomas
Verified customer
Asked: 2013-10-10
Answer
The products we sell, including anti-NEMO/IKK-gamma antibody A00874-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-10-10
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-NEMO/IKK-gamma antibody A00874-1. Let me know if you need anything else.
L. Carter
Verified customer
Asked: 2013-01-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-01-10
