Product Info Summary
SKU: | A01373-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IGFBP2 Antibody Picoband™
SKU/Catalog Number
A01373-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IGFBP2 Antibody Picoband™ catalog # A01373-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IGFBP2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01373-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat IGFBP2 recombinant protein (Position: E35-Q304).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01373-2 is reactive to IGFBP2 in Mouse, Rat
Applications
A01373-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
40 kDa
Calculated molecular weight
Background of IGFBP-2
The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Human IGFBP-2 cDNA encodes a 328 amino acid (aa) residue precursor protein with a putative 39 aa residue signal peptide that is processed to generate the 289 aa residue mature protein. IGFBP-2 contains an integrin receptor recognition sequence (RGD sequence) but lacks potential N-linked glycosylation sites. During development, IGFBP-2 is expressed in a number of tissues. The highest expression level is found in the central nervous system. In adults, high expression levels are also detected in the central nervous system and in a number of reproductive tissues. IGFBP-2 binds preferentially to IGF II, exhibiting a 2-10 fold higher affinity for IGF II than for IGF I.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
![A01373 2 IGFBP2 primary antibodies WB testing 1_1 A01373 2 IGFBP2 primary antibodies WB testing 1_1](https://www.bosterbio.com/media/catalog/product/A/0/A01373-2-IGFBP2-primary-antibodies-WB-testing-1_1.jpg)
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Figure 1. Western blot analysis of IGFBP2 using anti-IGFBP2 antibody (A01373-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse Neuro-2a whole cell lysates,
Lane 3: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP2 antigen affinity purified polyclonal antibody (Catalog # A01373-2) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP2 at approximately 40KD. The expected band size for IGFBP2 is at 35KD.
![A01373 2 IGFBP2 primary antibodies IHC testing 2_1 A01373 2 IGFBP2 primary antibodies IHC testing 2_1](https://www.bosterbio.com/media/catalog/product/A/0/A01373-2-IGFBP2-primary-antibodies-IHC-testing-2_1.jpg)
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Figure 2. IHC analysis of IGFBP2 using anti-IGFBP2 antibody (A01373-2).
IGFBP2 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IGFBP2 Antibody (A01373-2) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![A01373 2 IGFBP2 primary antibodies IHC testing 3_1 A01373 2 IGFBP2 primary antibodies IHC testing 3_1](https://www.bosterbio.com/media/catalog/product/A/0/A01373-2-IGFBP2-primary-antibodies-IHC-testing-3_1.jpg)
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Figure 3. IHC analysis of IGFBP2 using anti-IGFBP2 antibody (A01373-2).
IGFBP2 was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IGFBP2 Antibody (A01373-2) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![A01373 2 IGFBP2 primary antibodies IHC testing 4_1 A01373 2 IGFBP2 primary antibodies IHC testing 4_1](https://www.bosterbio.com/media/catalog/product/A/0/A01373-2-IGFBP2-primary-antibodies-IHC-testing-4_1.jpg)
Click image to see more details
Figure 4. IHC analysis of IGFBP2 using anti-IGFBP2 antibody (A01373-2).
IGFBP2 was detected in paraffin-embedded section of mouse kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IGFBP2 Antibody (A01373-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![A01373 2 IGFBP2 primary antibodies IHC testing 5_1 A01373 2 IGFBP2 primary antibodies IHC testing 5_1](https://www.bosterbio.com/media/catalog/product/A/0/A01373-2-IGFBP2-primary-antibodies-IHC-testing-5_1.jpg)
Click image to see more details
Figure 5. IHC analysis of IGFBP2 using anti-IGFBP2 antibody (A01373-2).
IGFBP2 was detected in paraffin-embedded section of rat brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IGFBP2 Antibody (A01373-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![A01373 2 IGFBP2 primary antibodies IHC testing 6_1 A01373 2 IGFBP2 primary antibodies IHC testing 6_1](https://www.bosterbio.com/media/catalog/product/A/0/A01373-2-IGFBP2-primary-antibodies-IHC-testing-6_1.jpg)
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Figure 6. IHC analysis of IGFBP2 using anti-IGFBP2 antibody (A01373-2).
IGFBP2 was detected in paraffin-embedded section of rat lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IGFBP2 Antibody (A01373-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![a01373 2 igfbp2 primary antibodies ihc testing 7 a01373 2 igfbp2 primary antibodies ihc testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a01373-2-igfbp2-primary-antibodies-ihc-testing-7.jpg)
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Figure 7. IF analysis of IGFBP2 using anti-IGFBP2 antibody (A01373-2).
IGFBP2 was detected in immunocytochemical section of NRK cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-IGFBP2 Antibody (A01373-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a01373 2 igfbp2 primary antibodies fcm testing 8 a01373 2 igfbp2 primary antibodies fcm testing 8](https://www.bosterbio.com/media/catalog/product/a/0/a01373-2-igfbp2-primary-antibodies-fcm-testing-8.jpg)
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Figure 8. Flow Cytometry analysis of RH35 cells using anti-IGFBP2 antibody (A01373-2).
Overlay histogram showing RH35 cells stained with A01373-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IGFBP2 Antibody (A01373-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For IGFBP2 (Source: Uniprot.org, NCBI)
Gene Name
IGFBP2
Full Name
Insulin-like growth factor-binding protein 2
Weight
Alternative Names
BP2; IBP2; IBP-2; IGF-binding protein 2; IGFBP2; IGFBP-2; IGF-BP53; insulin-like growth factor binding protein 2 (36kD); insulin-like growth factor binding protein 2, 36kDa; insulin-like growth factor-binding protein 2 IGFBP2 IBP2, IGF-BP53 insulin like growth factor binding protein 2 insulin-like growth factor-binding protein 2|IGF-binding protein 2|insulin-like growth factor binding protein 2, 36kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGFBP2, check out the IGFBP2 Infographic
![IGFBP2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGFBP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IGFBP2 Antibody Picoband™ (A01373-2)
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