Anti-HtrA3 Antibody Picoband™

Product Name

Anti-HtrA3 Antibody Picoband™

View all Htra3 Antibodies

SKU/Catalog Number

A05478

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HtrA3 Antibody Picoband™ catalog # A05478. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HtrA3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05478)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HtrA3, different from the related mouse sequence by four amino acids, and from the related rat sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05478 is reactive to Htra3 in Human

Applications

A05478 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

49 kDa

Calculated molecular weight

48608 MW

Background of Htra3

Human HtrA3 protease, which induces mitochondria-mediated apoptosis, can be a tumor suppressor and a potential therapeutic target in the treatment of cancer. It may also have a role in ovarian development, granulosa cell differentiation and luteinization. The long isoform, HTRA3L, contains 453 amino acids and has a predicted molecular mass of 49 kD. It contains an N-terminal signal peptide, followed by an insulin/IGF (see 147440)-binding domain, a Kazal-type S protease inhibitor domain, a trypsin protease domain, and a PDZ domain. The short isoform, HTRA3S, contains 357 amino acids and has a predicted molecular mass of 38 kD.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of HtrA3 using anti-HtrA3 antibody (A05478).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: PANC-1 whole Cell lysates,
Lane 2: HEPG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HtrA3 antigen affinity purified polyclonal antibody (Catalog # A05478) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HtrA3 at approximately 49KD. The expected band size for HtrA3 is at 49KD.

Gene/Protein Information For Htra3 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.4.21; EC 3.4.21.-; EC 3.4.21.108; High-temperature requirement factor A3; HTRA 3; HtrA serine peptidase 3; HTRA3; Pregnancy-related serine protease; probable serine protease HTRA3; Prsp; serine protease HTRA3; Tasp Htra3|2210021K23Rik, 9530081K03Rik, Prsp, Tasp|HtrA serine peptidase 3|serine protease HTRA3|high-temperature requirement factor A3|pregnancy-related serine protease|probable serine protease HTRA3|toll-associated serine protease

For more info on Htra3, check out the Htra3 Infographic

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In this infographic, you will see the following information for Htra3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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