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Product Info Summary
| SKU: | M00928-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Glutathione Synthetase Rabbit Monoclonal Antibody
View all Glutathione Synthetase Antibodies
SKU/Catalog Number
M00928-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Glutathione Synthetase Rabbit Monoclonal Antibody catalog # M00928-2. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glutathione Synthetase Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00928-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
20G11
Isotype
IgG
Immunogen
A synthesized peptide derived from human Glutathione Synthetase
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00928-2 is reactive to GSS in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
52 kDa
Calculated molecular weight
20082 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00928-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:100
Positive Control
WB: rat lung tissue, rat kidney tissue, mouse lung tissue, mouse kidney tissue, human 293T whole cell, human Hela whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GSS using anti-GSS antibody (M00928-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSS antigen affinity purified monoclonal antibody (M00928-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSS at approximately 52 kDa. The expected band size for GSS is at 52 kDa.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of Glutathione Synthetase expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For GSS (Source: Uniprot.org, NCBI)
Gene Name
GSS
Full Name
Glutathione synthetase
Weight
20082 MW
Superfamily
eukaryotic GSH synthase family
Alternative Names
ADP-ribosylation factor 6;ARF6; GSS GSHS, HEL-S-64p, HEL-S-88n glutathione synthetase glutathione synthetase|GSH synthetase|GSH-S|glutathione synthase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GSS, check out the GSS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GSS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glutathione Synthetase Rabbit Monoclonal Antibody (M00928-2)
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