Product Info Summary
SKU: | M00928 |
---|---|
Size: | 100 µl |
Reactive Species: | Dog, Human, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-Glutathione Synthetase (GSS) Mouse Monoclonal Antibody [Clone ID: OTI2F2]
View all Glutathione Synthetase Antibodies
SKU/Catalog Number
M00928
Size
100 µl
Description
Boster Bio GSS (Glutathione Synthetase) mouse monoclonal antibody, clone OTI2F2 (formerly 2F2). Catalog# M00928. Tested in FC, IF, WB. This antibody reacts with Human, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glutathione Synthetase (GSS) Mouse Monoclonal Antibody [Clone ID: OTI2F2] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00928)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI2F2
Isotype
IgG1
Immunogen
Full length human recombinant protein of human GSS (NP_000169) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00928 is reactive to GSS in Dog, Human, Mouse, Rat
Reconstitution
Calculated molecular weight
52.385kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00928 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells transfected with either GSS (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GSS antibody (M00928)
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Anti-GSS mouse monoclonal antibody (M00928) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GSS.
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSS.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GSS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:500).
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and GSS-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-GSS monoclonal antibody M00928
Protein Target Info & Infographic
Gene/Protein Information For GSS (Source: Uniprot.org, NCBI)
Gene Name
GSS
Full Name
Glutathione synthetase
Weight
52.385kDa
Superfamily
eukaryotic GSH synthase family
Alternative Names
EC 6.3.2.3; Glutathione synthase; glutathione synthetase; GSH synthetase; GSHS; GSH-S; MGC14098 GSS GSHS, HEL-S-64p, HEL-S-88n glutathione synthetase glutathione synthetase|GSH synthetase|GSH-S|glutathione synthase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GSS, check out the GSS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GSS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glutathione Synthetase (GSS) Mouse Monoclonal Antibody [Clone ID: OTI2F2] (M00928)
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