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Product Info Summary
| SKU: | M00780-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal Antibody
View all LAMP-1/CD107a Antibodies
SKU/Catalog Number
M00780-2
Size
100ul
Description
Boster Bio Anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal Antibody catalog # M00780-2. Tested in IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00780-2)
Host
Mouse
Contents
Purified at 1mg/mL in PBS, 50% glycerol, 5mM NaN3
Clonality
Monoclonal
Immunogen
amino acids 32-350 of the human LAMP1 precursor sequence in NP_005552.3 purified from E. coli
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00780-2 is reactive to Lamp1 in Human
Reconstitution
Calculated molecular weight
43865 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00780-2 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:10,000. IF/ICC and IHC: 1:2,000.
Validation Images & Assay Conditions
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Western blot analysis of different cell lysates using mouse mAb to LAMP1, M00780-2 dilution 1:10,000 in green. Cells were maintained under normal conditions (Ct), or treated with 50
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Immunofluorescent analysis of HeLa cells stained with mouse mAb to LAMP1, M00780-2, dilution 1:500 in red, and costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. The cells were treated with 50
Protein Target Info & Infographic
Gene/Protein Information For Lamp1 (Source: Uniprot.org, NCBI)
Gene Name
Lamp1
Full Name
Lysosome-associated membrane glycoprotein 1
Weight
43865 MW
Superfamily
LAMP family
Alternative Names
Lysosome-associated membrane glycoprotein 1;LAMP-1;Lysosome-associated membrane protein 1;120 kDa lysosomal membrane glycoprotein;CD107 antigen-like family member A;LGP-120;Lysosomal membrane glycoprotein A;LGP-A;P2B;CD107a;Lamp1;Lamp-1; LAMP1 CD107a, LAMPA, LGP120 lysosomal associated membrane protein 1 lysosome-associated membrane glycoprotein 1|CD107 -like family member A|LAMP-1|lysosome-associated membrane protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Lamp1, check out the Lamp1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Lamp1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal Antibody (M00780-2)
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No publications found for M00780-2
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Customer Q&As
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16 Customer Q&As for Anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal Antibody
Question
Is this M00780-2 anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody reactive to the isotypes of LAMP1?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
The immunogen of M00780-2 anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody is amino acids 32-350 of the human LAMP1 precursor sequence in NP_005552.3 purified from E. coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-26
Question
We have observed staining in human placenta. Are there any suggestions? Is anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
According to literature placenta does express LAMP1. According to Uniprot.org, LAMP1 is expressed in nephron tubule, mammary gland, brain, pancreas testis, placenta, plasma, liver, among other tissues. Regarding which tissues have LAMP1 expression, here are a few articles citing expression in various tissues:
Brain, Pancreas, and Testis, Pubmed ID: 15489334
Liver, Pubmed ID: 19159218, 24275569
Mammary gland, Pubmed ID: 14702039
Placenta, Pubmed ID: 17897319, 8517882, 2584229, 2243102, 8323299
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2020-03-11
Question
I was wanting to use to test anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody M00780-2 on human plasma for research purposes, then I may be interested in using anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody M00780-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
The products we sell, including anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody M00780-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-31
Question
Our lab used your anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody for WB on nephron tubule in the past. I am using human, and I plan to use the antibody for IF next. My question regards examining nephron tubule as well as placenta in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
I took a look at the website and datasheets of our anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody and I see that M00780-2 has been validated on human in both WB and IF. Thus M00780-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-29
Question
I was wanting to use your anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody for WB for human plasma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human plasma identification?
V. Krishna
Verified customer
Asked: 2019-10-28
Answer
As indicated on the product datasheet, M00780-2 anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody has been validated for IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human plasma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-28
Question
My question regards using your anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody for autophagic cell death studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
Thanks for your inquiry. This M00780-2 anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody is tested on green cells, hela cells. It is guaranteed to work for IF, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-18
Question
Is a blocking peptide available for product anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody (M00780-2)?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
We do provide the blocking peptide for product anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody (M00780-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-12
Question
Is there a BSA free version of anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody M00780-2 available?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody M00780-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-16
Question
Does anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody M00780-2 work for WB with plasma?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
According to the expression profile of plasma, LAMP1 is highly expressed in plasma. So, it is likely that anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody M00780-2 will work for WB with plasma.
Boster Scientific Support
Answered: 2019-06-26
Question
I have attached the WB image, lot number and protocol we used for plasma using anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody M00780-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-14
Question
We are currently using anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody M00780-2 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-29
Answer
The anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody (M00780-2) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-29
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for plasma using anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody M00780-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-04-18
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-18
Question
I see that the anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody M00780-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-03-23
Question
Would M00780-2 anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-10
Answer
It shows on the product datasheet, M00780-2 anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-10
Question
My question regarding product M00780-2, anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00780-2 anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-26
Question
We were well pleased with the WB result of your anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal antibody. However we have been able to see positive staining in placenta cell membrane using this antibody. Is that expected? Could you tell me where is LAMP1 supposed to be expressed?
J. Gonzalez
Verified customer
Asked: 2013-11-27
Answer
From what I have seen in literature, placenta does express LAMP1. Generally LAMP1 expresses in cell membrane. Regarding which tissues have LAMP1 expression, here are a few articles citing expression in various tissues:
Brain, Pancreas, and Testis, Pubmed ID: 15489334
Liver, Pubmed ID: 19159218, 24275569
Mammary gland, Pubmed ID: 14702039
Placenta, Pubmed ID: 17897319, 8517882, 2584229, 2243102, 8323299
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2013-11-27
