Anti-LAMP1 Monoclonal Antibody

Product Name

Anti-LAMP1 Monoclonal Antibody

View all LAMP-1/CD107a Antibodies

SKU/Catalog Number

M00780-1

Size

100ug

Form

liquid

Description

Boster Bio Anti-LAMP1 Monoclonal Antibody catalog # M00780-1. Tested in IP, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LAMP1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00780-1)

Host

Mouse

Contents

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

Ly1C6

Isotype

IgG1

Immunogen

Rat liver lysosomal membrane preparations

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~120kDa.

Reactive Species

M00780-1 is reactive to LAMP1 in Hamster, Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

165866 MW

Background of LAMP-1/CD107a

Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl¬terminal tyrosine ba based motif (YXXI) (1). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome (3). This stimulation has also been shown to have an associated release of histamine, leukotriene C (4) and prostaglandin D (2), which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells (1). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00780-1 is guaranteed for IP, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

Gene/Protein Information For LAMP1 (Source: Uniprot.org, NCBI)

Alternative Names

CD107, CD107a, LAMPA, LGP120, IgpA, Lysosome-associated membrane glycoprotein 1, CD107 antigen-like family member A, 120 kDa lysosomal membrane glycoprotein, Lamp-1 LAMP1 CD107a, LAMPA, LGP120 lysosomal associated membrane protein 1 lysosome-associated membrane glycoprotein 1|CD107 -like family member A|LAMP-1|lysosome-associated membrane protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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