Anti-Glutaminase/GLS Antibody Picoband® (monoclonal, 3G13)

Glutaminase antibody

Boster Bio Anti-Glutaminase/GLS Antibody Picoband® (monoclonal, 3G13) catalog # M01272-3. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: M01272-3
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-Glutaminase/GLS Antibody Picoband® (monoclonal, 3G13)

View all Glutaminase Antibodies

SKU/Catalog Number

M01272-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Glutaminase/GLS Antibody Picoband® (monoclonal, 3G13) catalog # M01272-3. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Glutaminase/GLS Antibody Picoband® (monoclonal, 3G13) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01272-3)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

3G13

Isotype

Mouse IgG2a

Immunogen

E.coli-derived human Glutaminase/GLS recombinant protein (Position: K396-N654).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M01272-3 is reactive to GLS in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

56-73 kDa

Calculated molecular weight

73.461kDa

Background of Glutaminase

This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01272-3 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human

Positive Control

WB: human HepG2 whole cell, human HEL whole cell, rat brain tissue, mouse brain tissue
IHC: human gall bladder adenosquamous carcinoma tissue, human appendiceal adenocarcinoma tissue, human breast cancer tissue, human liver cancer tissue
ICC/IF: SiHa cell
FCM: U937 cell

Validation Images & Assay Conditions

Gene/Protein Information For GLS (Source: Uniprot.org, NCBI)

Gene Name

GLS

Full Name

Glutaminase kidney isoform, mitochondrial

Weight

73.461kDa

Superfamily

glutaminase family

Alternative Names

Ubiquitin carboxyl-terminal hydrolase 7; Deubiquitinating enzyme 7; Herpesvirus-associated ubiquitin-specific protease; Ubiquitin thioesterase 7; Ubiquitin-specific-processing protease 7 GLS AAD20, CASGID, DEE71, EIEE71, GAC, GAM, GDPAG1, KGA, GLS glutaminase glutaminase kidney isoform, mitochondrial|K-glutaminase|L-glutamine amidohydrolase|glutaminase C|glutaminase, phosphate-activated

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GLS, check out the GLS Infographic

GLS infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GLS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M01272-3

100μg

$370
M01272-3-10ug

10μg sample (liquid)

$99
M01272-3-Biotin

100 μg Biotin conjugated

$570
M01272-3-Cy3

100 μg Cy3 conjugated

$570
M01272-3-Dylight488

100 μg Dylight488 conjugated

$570
M01272-3-Dylight550

100 μg Dylight550 conjugated

$570
M01272-3-Dylight594

100 μg Dylight594 conjugated

$570
M01272-3-FITC

100 μg FITC conjugated

$570
M01272-3-HRP

100 μg HRP conjugated

$570
M01272-3-APC

100 μg APC conjugated

$670
M01272-3-PE

100 μg PE conjugated

$670
M01272-3-iFluor647

100 μg iFluor647 conjugated

$670
M01272-3-carrier-free

Carrier Free

$370

More conjugates/formats

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M01272-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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