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Product Info Summary
| SKU: | A00042-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Caspase 8/CASP8 Antibody Picoband®
SKU/Catalog Number
A00042-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase 8/CASP8 Antibody Picoband® catalog # A00042-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase 8/CASP8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00042-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Caspase 8 recombinant protein (Position: Q389-D479).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00042-1 is reactive to CASP8 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55-60 kDa
Calculated molecular weight
55.391kDa
Background of Caspase-8
CASP8 is also known as CAP4, MACH or MCH5. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. In addtion, this protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00042-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human Hela whole cell, human SGC-7901 whole cell, rat thymus tissue, mouse spleen tissue, mouse thymus tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Caspase 8 using anti-Caspase 8 antibody (A00042-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SGC-7901 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 8 antigen affinity purified polyclonal antibody (Catalog # A00042-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase 8 at approximately 55KD. The expected band size for Caspase 8 is at 55KD.
Click image to see more details
Figure 1. Western blot analysis of Caspase 8 using anti-Caspase 8 antibody (A00042-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 8 antigen affinity purified polyclonal antibody (Catalog # A00042-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase 8 at approximately 55-60KD. The expected band size for Caspase 8 is at 55KD.
Protein Target Info & Infographic
Gene/Protein Information For CASP8 (Source: Uniprot.org, NCBI)
Gene Name
CASP8
Full Name
Caspase-8
Weight
55.391kDa
Superfamily
peptidase C14A family
Alternative Names
Caspase-8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH; Caspase-8 subunit p18; Caspase-8 subunit p10; CASP8; MCH5 CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5 caspase 8 caspase-8|FADD-homologous ICE/CED-3-like protease|FADD-like ICE|ICE-like apoptotic protease 5|MACH-alpha-1/2/3 protein|MACH-beta-1/2/3/4 protein|MORT1-associated ced-3 homolog|apoptotic cysteine protease|apoptotic protease Mch-5|caspase 8, apoptosis-related cysteine peptidase|caspase 8, apoptosis-related cysteine protease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP8, check out the CASP8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase 8/CASP8 Antibody Picoband® (A00042-1)
Hello CJ!
A00042-1 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhou L,Yu W,Jayabalan DS,Niesvizky R,Jaffrey SR,Huang X,Xu G.Caspase-8 Inhibition Prevents the Cleavage and Degradation of E3 Ligase Substrate Receptor Cereblon and Potentiates Its Biological Function.Front Cell Dev Biol.2020 Dec 17;8:605989.doi:10.3389/f
Species: Human
A00042-1 usage in article: APP:WB, SAMPLE:HEK293 CELL AND HELA CELL, DILUTION:NA
Inhibition of proliferation of prostate cancer cell line, PC-3, in vitro and in vivo using (%u2212)-gossypol
Protective effect of RIP and c-FLIP in preventing liver cancer cell apoptosis induced by TRAIL
Inhibitory activity of apogossypol in human prostate cancerin vitroandin vivo
Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir
Inhibition of proliferation of prostate cancer cell line, PC-3, in vitro and in vivo using (?)-gossypol
Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells
2-DG-Regulated RIP and c-FLIP Effect on Liver Cancer Cell Apoptosis Induced by TRAIL
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Customer Q&As
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16 Customer Q&As for Anti-Caspase 8/CASP8 Antibody Picoband®
Question
Is this A00042-1 anti-Caspase 8/CASP8 antibody reactive to the isotypes of CASP8?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
The immunogen of A00042-1 anti-Caspase 8/CASP8 antibody is E. coli-derived human Caspase 8 recombinant protein (Position: Q389-D479). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-05
Question
My colleagues were happy with the WB result of your anti-Caspase 8/CASP8 antibody. However we have seen positive staining in small intestine peyer's patch cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP8 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
From literature, small intestine peyer's patch does express CASP8. Generally CASP8 expresses in cytoplasm. Regarding which tissues have CASP8 expression, here are a few articles citing expression in various tissues:
B-cell, and Thymus, Pubmed ID: 8681376
Leukocyte, Pubmed ID: 12010809, 15489334
T-cell, Pubmed ID: 8755496
Boster Scientific Support
Answered: 2020-04-21
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for small intestine peyer's patch using anti-Caspase 8/CASP8 antibody A00042-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-14
Question
We have seen staining in rat leukocyte. What should we do? Is anti-Caspase 8/CASP8 antibody supposed to stain leukocyte positively?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
From what I have seen in literature leukocyte does express CASP8. From what I have seen in Uniprot.org, CASP8 is expressed in small intestine peyer's patch, b-cell thymus, t-cell, leukocyte, among other tissues. Regarding which tissues have CASP8 expression, here are a few articles citing expression in various tissues:
B-cell, and Thymus, Pubmed ID: 8681376
Leukocyte, Pubmed ID: 12010809, 15489334
T-cell, Pubmed ID: 8755496
Boster Scientific Support
Answered: 2020-02-04
Question
Do you have a BSA free version of anti-Caspase 8/CASP8 antibody A00042-1 available?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Caspase 8/CASP8 antibody A00042-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-27
Question
Would A00042-1 anti-Caspase 8/CASP8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
You can see on the product datasheet, A00042-1 anti-Caspase 8/CASP8 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-27
Question
I would like using your anti-Caspase 8/CASP8 antibody for apoptotic process studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
We appreciate your inquiry. This A00042-1 anti-Caspase 8/CASP8 antibody is validated on human hela, hela whole cell lysates, rat thymus tissue, mouse thymus tissue, spleen tissue. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-24
Question
My question regarding product A00042-1, anti-Caspase 8/CASP8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00042-1 anti-Caspase 8/CASP8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-21
Question
We are currently using anti-Caspase 8/CASP8 antibody A00042-1 for human tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
The anti-Caspase 8/CASP8 antibody (A00042-1) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-03
Question
Is a blocking peptide available for product anti-Caspase 8/CASP8 antibody (A00042-1)?
Verified Customer
Verified customer
Asked: 2017-06-22
Answer
We do provide the blocking peptide for product anti-Caspase 8/CASP8 antibody (A00042-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-06-22
Question
My question regards to test anti-Caspase 8/CASP8 antibody A00042-1 on human small intestine peyer's patch for research purposes, then I may be interested in using anti-Caspase 8/CASP8 antibody A00042-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Rodriguez
Verified customer
Asked: 2017-02-10
Answer
The products we sell, including anti-Caspase 8/CASP8 antibody A00042-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-02-10
Question
I was wanting to use your anti-Caspase 8/CASP8 antibody for ELISA for human small intestine peyer's patch on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human small intestine peyer's patch identification?
P. Huang
Verified customer
Asked: 2014-11-27
Answer
As indicated on the product datasheet, A00042-1 anti-Caspase 8/CASP8 antibody has been validated for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human small intestine peyer's patch in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-11-27
Question
I see that the anti-Caspase 8/CASP8 antibody A00042-1 works with ELISA, what is the protocol used to produce the result images on the product page?
M. Yang
Verified customer
Asked: 2014-11-07
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-11-07
Question
Will anti-Caspase 8/CASP8 antibody A00042-1 work for ELISA with small intestine peyer's patch?
D. Miller
Verified customer
Asked: 2014-01-07
Answer
According to the expression profile of small intestine peyer's patch, CASP8 is highly expressed in small intestine peyer's patch. So, it is likely that anti-Caspase 8/CASP8 antibody A00042-1 will work for ELISA with small intestine peyer's patch.
Boster Scientific Support
Answered: 2014-01-07
Question
See attached the WB image, lot number and protocol we used for small intestine peyer's patch using anti-Caspase 8/CASP8 antibody A00042-1. Please let me know if you require anything else.
J. Miller
Verified customer
Asked: 2013-12-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-12-12
Question
Our lab used your anti-Caspase 8/CASP8 antibody for WB on leukocyte in the past. I am using human, and We intend to use the antibody for ELISA next. I would like examining leukocyte as well as t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
L. Edwards
Verified customer
Asked: 2013-10-29
Answer
I viewed the website and datasheets of our anti-Caspase 8/CASP8 antibody and I see that A00042-1 has been validated on human in both WB and ELISA. Thus A00042-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-10-29
