Anti-Glucocorticoid Receptor Nr3c1 Monoclonal Antibody (BuGR2)

Product Name

Anti-Glucocorticoid Receptor Nr3c1 Monoclonal Antibody (BuGR2)

View all NR3C1 Antibodies

SKU/Catalog Number

M00503-1

Size

100µg

Form

Lyophilized

Description

Boster Bio Anti-Glucocorticoid Receptor Nr3c1 Monoclonal Antibody (BuGR2) (Catalog# M00503-1). Tested in Flow Cytometry, ICC, IHC-P, IP, WB, Gel Shift application(s). This antibody reacts with Human, Mouse, Rat, Guinea pig, Rabbit, Sheep, Yeast.

Storage & Handling

Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Cite This Product

Anti-Glucocorticoid Receptor Nr3c1 Monoclonal Antibody (BuGR2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00503-1)

Host

Mouse

Contents

Lyophilized from PBS, pH 7.2, containing 0.05% sodium azide.

Clonality

Monoclonal

Clone Number

BuGR2

Isotype

IgG2

Immunogen

Partially purified rat GR (glucocorticoid receptor)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M00503-1 is reactive to NR3C1 in Guinea pig, Human, Mouse, Rabbit, Rat, Sheep, Yeast

Applications

M00503-1 is guaranteed for Flow Cytometry, IP, IHC-P, ICC, WB, Gel Shift Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

29159 MW

Background of NR3C1

Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DR and DP. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36kDa) and a chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (paraffin sections, 5µg/ml)
Western Blot (5µg/ml). Detects a band of ~97kDa.
Immunocytochemical staining of GR in L929 cells results in staining of both the cytoplasm and nucleus, even in the presence of hormone.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Nr3c1 using anti-Nr3c1 antibody (M00503-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nr3c1 antigen affinity purified polyclonal antibody (Catalog # M00503-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.

Gene/Protein Information For NR3C1 (Source: Uniprot.org, NCBI)

Alternative Names

GCR; glucocorticoid receptor; GR; GRGCCR; GRLNuclear receptor subfamily 3 group C member 1; NR3C1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); nuclear receptor subfamily 3, group C, member 1 NR3C1 GCCR, GCR, GCRST, GR, GRL nuclear receptor subfamily 3 group C member 1 glucocorticoid receptor|glucocorticoid nuclear receptor variant 1|nuclear receptor subfamily 3 group C member 1 variant hGR-B(54)|nuclear receptor subfamily 3 group C member 1 variant hGR-B(77)|nuclear receptor subfamily 3 group C member 1 variant hGR-B(93)|nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)

For more info on NR3C1, check out the NR3C1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NR3C1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the NR3C1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis