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Product Info Summary
| SKU: | EK1881 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Human CTRP9 ELISA Kit PicoKine®
View all CTRP9/C1qTNF9 ELISA kits
SKU/Catalog Number
EK1881
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CTRP9 ELISA Kit PicoKine® (96 Tests). Quantitate Human C1QTNF9 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CTRP9 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1881)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q20-P333
Sensitivity
<10 pg/ml
Assay Range
46.9 pg/ml - 3,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1881 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1881 is reactive to C1QTNF9 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK1881 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CTRP9/C1qTNF9
The C1QTNF9 gene is conserved in chimpanzee, Rhesus monkey, and zebrafish. This gene is mapped to 13q12.12. 42 organisms have orthologs with human gene C1QTNF9.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1881-CAP | Anti-Human C1QTNF9 Pre-coated 96-well strip microplate | 1 |
| EK1881-ST | Human C1QTNF9 Standard | 2 vials, 10 ng/tube |
| EK1881-DA | Human C1QTNF9 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 46.9 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 |
| O.D. | 0.007 | 0.026 | 0.069 | 0.162 | 0.354 | 0.799 | 1.518 | 2.021 |
Data Example Images
Click image to see more details
Human CTRP9 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CTRP9/C1qTNF9, For serum, dilution ratio is 1:1 and concentration is around 400 pg/ml.
For plasma, dilution ratio is 1:1 and concentration is around 150 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CTRP9 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 65 | 280 | 1368 | 73 | 291 | 1425 |
| Standard deviation | 3.19 | 14.28 | 79.34 | 5.18 | 22.12 | 111.15 |
| CV (%) | 4.9% | 5.1% | 5.8% | 7.1% | 7.6% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CTRP9/C1qTNF9 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 65 | 59 | 68 | 62 | 63 | 3.35 | 5.3% |
| Sample 2 | 280 | 327 | 277 | 285 | 292 | 20.26 | 6.9% |
| Sample 3 | 1368 | 1367 | 1278 | 1292 | 1326 | 41.54 | 3.1% |
Protein Target Info & Infographic
Gene/Protein Information For C1QTNF9 (Source: Uniprot.Org, NCBI)
Gene Name
C1QTNF9
Full Name
Complement C1q and tumor necrosis factor-related protein 9
Weight
34.566kDa
Alternative Names
Complement C1q and tumor necrosis factor-related protein 9A; Complement C1q and tumor necrosis factor-related protein 9; C1QTNF9; C1QTNF9A; UNQ6503; PRO21380 C1qtnf9|9130217G22Rik, Adif1, CTR, CTRP9, Ciqtnf9|C1q and tumor necrosis factor related protein 9|complement C1q and tumor necrosis factor-related protein 9|adiponectin f1|complement C1q tumor necrosis factor-related protein 9
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on C1QTNF9, check out the C1QTNF9 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for C1QTNF9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CTRP9 ELISA Kit PicoKine® (EK1881)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Human CTRP9 ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for CTRP9 ELISA kit? I used your CTRP9 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CTRP9 even though the O.D. values are not very high?
B. Parker
Verified customer
Asked: 2019-06-02
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CTRP9 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CTRP9 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-06-02
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CTRP9 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
P. Edwards
Verified customer
Asked: 2019-04-10
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CTRP9 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-04-10
Question
Q: how to analyze ELISA data? I have obtained CTRP9 level in serum.
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-01-21
Question
Q: how to thaw whole blood sample for CTRP9 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-08-11
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CTRP9 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-08-11
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
P. Phillips
Verified customer
Asked: 2018-02-05
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-02-05
Question
Q: is it okay to use EDTA plasma as samples in Human CTRP9 Picokine® ELISA Kit (Catalog # EK1881)?
Verified Customer
Verified customer
Asked: 2015-02-07
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CTRP9 causing disruption of its protein structure. CTRP9 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CTRP9 ELISA, treating samples with various anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2015-02-07
