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Product Info Summary
| SKU: | M00503-2 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-NR3C1
SKU/Catalog Number
M00503-2
Size
50 µl
Description
Boster Bio Anti-NR3C1 (Catalog # M00503-2). Tested in WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-NR3C1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00503-2)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
2153CT307.32.80
Isotype
IgG1,κ
Immunogen
This antibody is generated from a mouse immunized with a recombinant protein from human.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00503-2 is reactive to NR3C1 in Human, Mouse
Reconstitution
Calculated molecular weight
85659 Da
Background of NR3C1
Receptor for glucocorticoids (GC) (PubMed:27120390). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00503-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-NR3C1 at dilution
Lane 1: DU145 whole cell lysate
Lane 2: U-251MG whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 86 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-NR3C1 at dilution
Lane 1: HeLa whole cell lysate
Lane 2: A549 whole cell lysate
Lane 3: CCRF-CEM whole cell lysate
Lane 4: CHO whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 86 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For NR3C1 (Source: Uniprot.org, NCBI)
Gene Name
NR3C1
Full Name
Glucocorticoid receptor
Weight
85659 Da
Superfamily
nuclear hormone receptor family
Alternative Names
Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1, NR3C1, GRL NR3C1 GCCR, GCR, GCRST, GR, GRL nuclear receptor subfamily 3 group C member 1 glucocorticoid receptor|glucocorticoid nuclear receptor variant 1|nuclear receptor subfamily 3 group C member 1 variant hGR-B(54)|nuclear receptor subfamily 3 group C member 1 variant hGR-B(77)|nuclear receptor subfamily 3 group C member 1 variant hGR-B(93)|nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NR3C1, check out the NR3C1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NR3C1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NR3C1 (M00503-2)
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