Product Info Summary
SKU: | PA1492 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-GCLC Antibody
SKU/Catalog Number
PA1492
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GCLC Antibody catalog # PA1492. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GCLC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1492)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human GCLC, different from the related rat and mouse sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1492 is reactive to GCLC in Human, Mouse, Rat
Applications
PA1492 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
20 kDa
Calculated molecular weight
72766 MW
Background of GCLC
GCLC, Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-GCLC antibody, PA1492, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: Rat Heart Tissue Lysate
Lane 4: Rat Lung Tissue Lysate
Click image to see more details
Anti-GCLC antibody, PA1492, IHC(P)
IHC(P): Human Rectal Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For GCLC (Source: Uniprot.org, NCBI)
Gene Name
GCLC
Full Name
Glutamate--cysteine ligase catalytic subunit
Weight
72766 MW
Superfamily
glutamate--cysteine ligase type 3 family
Alternative Names
EC 6.3.2.2; gamma-ECS; Gamma-glutamylcysteine synthetase; GCS heavy chain; GCS; GLCL; GLCLCGCL; glutamate--cysteine ligase catalytic subunit; glutamate-cysteine ligase, catalytic subunit GCLC GCL, GCS, GLCL, GLCLC glutamate-cysteine ligase catalytic subunit glutamate--cysteine ligase catalytic subunit|GCS heavy chain|gamma-ECS|gamma-glutamylcysteine synthetase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GCLC, check out the GCLC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GCLC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GCLC Antibody (PA1492)
Hello CJ!
PA1492 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Aldosterone Induces DNA Damage and Activation of Nrf2 Mainly in Tubuli of Mouse Kidneys
Zhilei Mao,Shushu Li,Lina Zhang,Mengmeng Yao,Zhu Zhou,Minjian Chen,"The mTOR/GCLc/GSH Pathway Mediates the Dose-Dependent Bidirectional Regulation of ROS Induced by TiO2 NPs in Neurogenic Cells",Oxidative Medicine and Cellular Longevity,vol.2019,Article ID 7621561,14 pages,2019.https://doi.org/10.1155/2019/7621561
Species: Human
PA1492 usage in article: APP:WB, SAMPLE:SH-SY5Y CELL, DILUTION:NA
Electroacupuncture alleviates cerebral ischemia and reperfusion injury via modulation of the ERK1/2 signaling pathway
Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling
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