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Product Info Summary
| SKU: | M01722 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GCLC Rabbit Monoclonal Antibody
SKU/Catalog Number
M01722
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GCLC Rabbit Monoclonal Antibody catalog # M01722. Tested in WB application. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GCLC Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01722)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABBG-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GCLC
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01722 is reactive to GCLC in Human, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
73 kDa
Calculated molecular weight
73 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01722 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: human Hela whole cell, human 293T whole cell, human HepG2 whole cell, human MCF-7 whole cell, rat kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GCLC using anti-GCLC antibody (M01722).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCLC antigen affinity purified monoclonal antibody (Catalog # M01722) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCLC at approximately 73 kDa. The expected band size for GCLC is at 73 kDa.
Protein Target Info & Infographic
Gene/Protein Information For GCLC (Source: Uniprot.org, NCBI)
Gene Name
GCLC
Full Name
Glutamate--cysteine ligase catalytic subunit
Weight
73 kDa
Superfamily
glutamate--cysteine ligase type 3 family
Alternative Names
Glutamate--cysteine ligase catalytic subunit;6.3.2.2;GCS heavy chain;Gamma-ECS;Gamma-glutamylcysteine synthetase;GCLC;GLCL, GLCLC; GCLC GCL, GCS, GLCL, GLCLC glutamate-cysteine ligase catalytic subunit glutamate--cysteine ligase catalytic subunit|GCS heavy chain|gamma-ECS|gamma-glutamylcysteine synthetase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GCLC, check out the GCLC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GCLC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GCLC Rabbit Monoclonal Antibody (M01722)
Hello CJ!
M01722 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhilei Mao,Shushu Li,Lina Zhang,Mengmeng Yao,Zhu Zhou,Minjian Chen,"The mTOR/GCLc/GSH Pathway Mediates the Dose-Dependent Bidirectional Regulation of ROS Induced by TiO2 NPs in Neurogenic Cells",Oxidative Medicine and Cellular Longevity,vol.2019,Article ID 7621561,14 pages,2019.https://doi.org/10.1155/2019/7621561
Species: Human
M01722 usage in article: APP:WB, SAMPLE:SH-SY5Y CELL, DILUTION:NA
Electroacupuncture alleviates cerebral ischemia and reperfusion injury via modulation of the ERK1/2 signaling pathway
Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling
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