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Product Info Summary
| SKU: | A00598 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Cytochrome P450 1A2/Cyp1a2 Antibody Picoband®
View all Cytochrome P450 1A2 Antibodies
SKU/Catalog Number
A00598
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cytochrome P450 1A2/Cyp1a2 Antibody Picoband® catalog # A00598. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome P450 1A2/Cyp1a2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00598)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Cytochrome P450 1A2/Cyp1a2 recombinant protein (Position: H184-K513).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00598 is reactive to Cyp1a2 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
58 kDa
Calculated molecular weight
58.407kDa
Background of Cytochrome P450 1A2
CYP1A2 (Cytochrome P450, Subfamily I, Polypeptide 2) is a member of the cytochrome P450 mixed-function oxidase system and is involved in the metabolism of xenobiotics in the body. CYP1A2 is a member of the cytochrome P450 superfamily of enzymes. In humans, the CYP1A2 enzyme is encoded by the CYP1A2 gene. CYP1A2 localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The CYP1A2 gene encodes a P450 enzyme involved in O-deethylation of phenacetin. Ikeya et al. (1989) found that the human CYP1A2 gene spans almost 7.8 kb and contains 7 exons. The CYP1A2 gene is mapped on 15q24.1. CYP1A2 accounts for nearly 15% of the cytochrome P450 in the human liver. CYP1A2 displays higher activity in men than in women, and is inhibited by oral contraceptives. Inducers of CYP1A2 include cruciferous vegetables. Cigarette smoking has also been shown to increase CYP1A2 activity. Expression of CYP1A2 appears to be induced by various dietary constituents.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00598 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat liver tissue, mouse liver tissue
FCM: HEPA1-6 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cytochrome P450 1A2/Cyp1a2 using anti-Cytochrome P450 1A2/Cyp1a2 antibody (A00598).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytochrome P450 1A2/Cyp1a2 antigen affinity purified polyclonal antibody (Catalog # A00598) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytochrome P450 1A2/Cyp1a2 at approximately 58KD. The expected band size for Cytochrome P450 1A2/Cyp1a2 is at 58KD.
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Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-Cytochrome P450 1A2/Cyp1a2 antibody (A00598).
Overlay histogram showing HEPA1-6 cells stained with A00598 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytochrome P450 1A2/Cyp1a2 Antibody (A00598, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Cyp1a2 (Source: Uniprot.org, NCBI)
Gene Name
Cyp1a2
Full Name
Cytochrome P450 1A2
Weight
58.407kDa
Superfamily
cytochrome P450 family
Alternative Names
Vesicle-fusing ATPase; N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion protein; Vesicular-fusion protein NSF; NSF CYP1A2 CP12, CYPIA2, P3-450, P450(PA) cytochrome P450 family 1 subfamily A member 2 cytochrome P450 1A2|P450 form 4|aryl hydrocarbon hydroxylase|cholesterol 25-hydroxylase|cytochrome P(3)450|cytochrome P450 4|cytochrome P450, family 1, subfamily A, polypeptide 2|cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2|cytochrome P450-P3|dioxin-inducible P3-450|flavoprotein-linked monooxygenase|hydroperoxy icosatetraenoate dehydratase|microsomal monooxygenase|xenobiotic monooxygenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cyp1a2, check out the Cyp1a2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cyp1a2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytochrome P450 1A2/Cyp1a2 Antibody Picoband® (A00598)
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