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Product Info Summary
| SKU: | M01722-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GCLC Mouse Monoclonal Antibody [Clone ID: OTI1A3]
SKU/Catalog Number
M01722-1
Size
100 µl
Description
Boster Bio GCLC mouse monoclonal antibody, clone OTI1A3 (formerly 1A3). Catalog# M01722-1. Tested in IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GCLC Mouse Monoclonal Antibody [Clone ID: OTI1A3] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01722-1)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1A3
Isotype
IgG1
Immunogen
Full length human recombinant protein of human GCLC (NP_001489) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01722-1 is reactive to GCLC in Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
72.766kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01722-1 is guaranteed for IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GCLC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GCLC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-GCLC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCLC (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCLC.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GCLC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Protein Target Info & Infographic
Gene/Protein Information For GCLC (Source: Uniprot.org, NCBI)
Gene Name
GCLC
Full Name
Glutamate--cysteine ligase catalytic subunit
Weight
72.766kDa
Superfamily
glutamate--cysteine ligase type 3 family
Alternative Names
EC 6.3.2.2; gamma-ECS; Gamma-glutamylcysteine synthetase; GCS heavy chain; GCS; GLCL; GLCLCGCL; glutamate--cysteine ligase catalytic subunit; glutamate-cysteine ligase, catalytic subunit GCLC GCL, GCS, GLCL, GLCLC glutamate-cysteine ligase catalytic subunit glutamate--cysteine ligase catalytic subunit|GCS heavy chain|gamma-ECS|gamma-glutamylcysteine synthetase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GCLC, check out the GCLC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GCLC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GCLC Mouse Monoclonal Antibody [Clone ID: OTI1A3] (M01722-1)
Hello CJ!
M01722-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhilei Mao,Shushu Li,Lina Zhang,Mengmeng Yao,Zhu Zhou,Minjian Chen,"The mTOR/GCLc/GSH Pathway Mediates the Dose-Dependent Bidirectional Regulation of ROS Induced by TiO2 NPs in Neurogenic Cells",Oxidative Medicine and Cellular Longevity,vol.2019,Article ID 7621561,14 pages,2019.https://doi.org/10.1155/2019/7621561
Species: Human
M01722-1 usage in article: APP:WB, SAMPLE:SH-SY5Y CELL, DILUTION:NA
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