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Product Info Summary
| SKU: | M00227-5 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human, Monkey |
| Host: | Rabbit |
| Application: | ChIP, IP, IHC, ICC, WB |
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Product info
Product Name
Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114
SKU/Catalog Number
M00227-5
Size
100uL
Form
Liquid
Description
Boster Bio Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114 (Catalog # M00227-5). Tested in WB, IP, ChIP, ICC, IHC applications. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227-5)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM114
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of GAPDH
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to GAPDH. RM114 shows strong reactivity to Human/Monkey GAPDH. RM114 shows weaker reactivity to Mouse, Goat GAPDH and may require higher antibody dilution usage.
Reactive Species
M00227-5 is reactive to GAPDH in Human, Monkey
Reconstitution
Calculated molecular weight
36053 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00227-5 is guaranteed for ChIP, IP, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (WB): 1:1000 dilution
Immunoprecipitation (IP): 1:200 dilution
Chromatin IP (ChIP): 1:200 dilution
Immunocytochemistry (ICC): 1:200 dilution
Immunohistochemistry (IHC): 1:200 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western blot of A431 cells, using Clone RM114 at 1/1000 dilution. A GAPDH band showed at the predicted MW (~36 kDa).
Click image to see more details
Figure 2. ICC result
Immunocytochemical staining of HeLa cells, using anti-GAPDH Clone RM114 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).
Protein Target Info & Infographic
Gene/Protein Information For GAPDH (Source: Uniprot.org, NCBI)
Gene Name
GAPDH
Full Name
Glyceraldehyde-3-phosphate dehydrogenase
Weight
36053 MW
Superfamily
glyceraldehyde-3-phosphate dehydrogenase family
Alternative Names
Glyceraldehyde-3-phosphate dehydrogenase;GAPDH;1.2.1.12;Peptidyl-cysteine S-nitrosylase GAPDH;2.6.99.-;GAPDH;GAPD;CDABP0047, OK/SW-cl.12; GAPDH G3PD, GAPD, HEL-S-162eP glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase|OCAS, p38 component|Oct1 coactivator in S phase, 38 Kd component|aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GAPDH, check out the GAPDH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GAPDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114 (M00227-5)
Hello CJ!
M00227-5 has been cited in 127 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
(-)-Epigallocatechin-3-gallate induces interferon-λ2 expression to anti-influenza A virus in human bronchial epithelial cells (BEAS-2B) through p38 MAPK signaling pathway
Circular RNA COL1A2 promotes angiogenesis via regulating miR-29b/VEGF axis in diabetic retinopathy
Xie R,Liu J,Yu X,Li C,Wang Y,Yang W,Hu J,Liu P,Sui H,Liang P,Huang X,Wang L,Bai Y,Xue Y,Zhu J,Fang T.ANXA2 Silencing Inhibits Proliferation, Invasion, and Migration in Gastric Cancer Cells.J Oncol.2019 May 2;2019:4035460.doi:10.1155/2019/4035460.PMID:31186633;PMCID:PMC6521490.
Species: Human
M00227-5 usage in article: APP:WB, SAMPLE:SGC-7901 CELL,MKN-45 CELL,BGC-823 CELL AND AGS CELL, DILUTION:NA
Tibatan MA,Önay Uçar E.Long non-coding RNA NKILA regulates expression of HSP90α, NF-κB and β-catenin proteins in the MCF-7 breast cancer cell line.Mol Biol Rep.2021 Jun 14.doi:10.1007/s11033-021-06482-y.Epub ahead of print.PMID:34125329.
Species: Human
M00227-5 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION:NA
Deng S,Zhang Y,Liao Z,Huang J,Huang R,Li Z.S100A4 plays a key role in TRPV3 ion channel expression and its electrophysiological function.Neurosci Lett.2021 May 28:135999.doi:10.1016/j.neulet.2021.135999.Epub ahead of print.PMID:34058292.
Species: Human
M00227-5 usage in article: APP:WB, SAMPLE:HEK293 T CELL, DILUTION:NA
Bin Wang,Jinyu Li,Mi Bao,Runji Chen,Haiyan Li,Binger Lu,Meixin Chen,Danmei Huang,Yanmei Zhang,Fenfei Gao,Ganggang Shi,"Melatonin Attenuates Diabetic Myocardial Microvascular Injury through Activating the AMPK/SIRT1 Signaling Pathway",Oxidative Medicine and Cellular Longevity,vol.2021,Article ID 8882130,15 pages,2021.https://doi.org/10.1155/2021/8882130
Species: Mouse
M00227-5 usage in article: APP:WB, SAMPLE:CMECS, DILUTION:NA
Chen X,Xu H,Hou J,Wang H,Zheng Y,Li H,Cai H,Han X,Dai J.Epithelial cell senescence induces pulmonary fibrosis through Nanog-mediated fibroblast activation.Aging (Albany NY).2019 Dec 31;12(1):242-259.doi:10.18632/aging.102613.Epub 2019 Dec 31.PMID:31891567
Species: Human,Mouse
M00227-5 usage in article: APP:WB, SAMPLE:MLE-12 CELL, DILUTION:NA
She Y,Sun J,Hou P,Fang P, Zhang Z. Time-restricted feeding attenuates gluconeogenic activity through inhibition of PGC-1α expression and activity. Physiol Behav. 2021 Mar 15;231:113313. doi: 10.1016/j.physbeh.2021.113313. Epub 2021 Jan 4.PMID:33412190.
Species: Mouse
M00227-5 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA
Gang-Zhu Xu,Yang Xue,Si-Qi Wei,Jia-Heng Li,Richard J. Traub,Mao-De Wang,Dong-Yuan Cao,Valproate reverses stress-induced somatic hyperalgesia and visceral hypersensitivity by up-regulating spinal 5-HT2C receptor expression in female rats,Neuropharmacology,
Species: Rat
M00227-5 usage in article: APP:WB, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:4000
Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
M00227-5 usage in article: APP:WB, SAMPLE:MSC-LIPO, DILUTION:NA
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114
Question
See below the WB image, lot number and protocol we used for cajal-retzius cell fetal brain cortex using anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-21
Question
Would anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5 work for IP with cajal-retzius cell fetal brain cortex?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
According to the expression profile of cajal-retzius cell fetal brain cortex, GAPDH is highly expressed in cajal-retzius cell fetal brain cortex. So, it is likely that anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5 will work for IP with cajal-retzius cell fetal brain cortex.
Boster Scientific Support
Answered: 2020-03-20
Question
Does M00227-5 anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
It shows on the product datasheet, M00227-5 anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-30
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cajal-retzius cell fetal brain cortex using anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-20
Question
Is this M00227-5 anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 reactive to the isotypes of GAPDH?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
The immunogen of M00227-5 anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 is A peptide corresponding to the C-terminus of GAPDH. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-19
Question
I was wanting to use your anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 for IP for mouse cajal-retzius cell fetal brain cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cajal-retzius cell fetal brain cortex identification?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
You can see on the product datasheet, M00227-5 anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 has been tested for IP, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cajal-retzius cell fetal brain cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-17
Question
I see that the anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-08
Question
My lab would like using your anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 for killing by host of symbiont cells studies. Has this antibody been tested with western blotting on a431 cells? We would like to see some validation images before ordering.
E. Wu
Verified customer
Asked: 2019-06-28
Answer
I appreciate your inquiry. This M00227-5 anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 is tested on a431 cells, hela cells. It is guaranteed to work for IP, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-28
Question
I am interested in to test anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5 on mouse cajal-retzius cell fetal brain cortex for research purposes, then I may be interested in using anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-04-26
Answer
The products we sell, including anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-26
Question
Do you have a BSA free version of anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5 available?
Verified Customer
Verified customer
Asked: 2019-01-31
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-31
Question
Can you help my question with product M00227-5, anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-04-13
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00227-5 anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-13
Question
Is a blocking peptide available for product anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 (M00227-5)?
Verified Customer
Verified customer
Asked: 2017-12-07
Answer
We do provide the blocking peptide for product anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 (M00227-5). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-12-07
Question
Our team were happy with the WB result of your anti-GAPDH Rabbit Monoclonal antibody, Clone
Z. Collins
Verified customer
Asked: 2016-09-12
Answer
From literature, leukemic t-cell does express GAPDH. Generally GAPDH expresses in cytoplasm, cytosol. Regarding which tissues have GAPDH expression, here are a few articles citing expression in various tissues:
Astrocytoma, Pubmed ID: 10944468
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 7498159
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 2987855, 24275569
Lung, Pubmed ID: 3664468
Lymphoblast, Pubmed ID: 14654843
Muscle, Pubmed ID: 1193541, 11724794
Placenta, Pubmed ID: 1924305
Boster Scientific Support
Answered: 2016-09-12
Question
We are currently using anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 M00227-5 for human tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
S. Krishna
Verified customer
Asked: 2016-06-17
Answer
The anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 (M00227-5) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-06-17
Question
We have been able to see staining in human cajal-retzius cell fetal brain cortex. Any tips? Is anti-GAPDH Rabbit Monoclonal antibody, Clone#RM114 supposed to stain cajal-retzius cell fetal brain cortex positively?
K. Zhao
Verified customer
Asked: 2015-08-28
Answer
From what I have seen in literature cajal-retzius cell fetal brain cortex does express GAPDH. From what I have seen in Uniprot.org, GAPDH is expressed in smooth muscle tissue, liver, lung, placenta, astrocytoma, leukemia, colon adenocarcinoma, eye, kidney, lung, lymph placenta, muscle, platelet, prostatic carcinoma, brain, cajal-retzius cell fetal brain cortex, heart, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have GAPDH expression, here are a few articles citing expression in various tissues:
Astrocytoma, Pubmed ID: 10944468
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 7498159
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 2987855, 24275569
Lung, Pubmed ID: 3664468
Lymphoblast, Pubmed ID: 14654843
Muscle, Pubmed ID: 1193541, 11724794
Placenta, Pubmed ID: 1924305
Boster Scientific Support
Answered: 2015-08-28
Question
We ordered your anti-GAPDH Rabbit Monoclonal antibody, Clone
K. Kulkarni
Verified customer
Asked: 2015-01-23
Answer
I have checked the website and datasheets of our anti-GAPDH Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2015-01-23
