Product Info Summary
SKU: | M00256-1 |
---|---|
Size: | 100 µl |
Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-HDAC1 Mouse Monoclonal Antibody [Clone ID: OTI5F9]
SKU/Catalog Number
M00256-1
Size
100 µl
Description
Boster Bio Mouse monoclonal anti-HDAC1 antibody, clone 5F9, Loading, clone OTI5F9 (formerly 5F9). Catalog# M00256-1. Tested in FC, IF, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-HDAC1 Mouse Monoclonal Antibody [Clone ID: OTI5F9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00256-1)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI5F9
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human HDAC1 (NP_004955) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00256-1 is reactive to HDAC1 in Dog, Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
55.103kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00256-1 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC1.
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Western blot analysis of extracts (10ug) from 9 different cell lines by using anti-HDAC1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:1000).
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HEK293T cells transfected with either HDAC1 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HDAC1 antibody (M00256-1)
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Anti-HDAC1 mouse monoclonal antibody (M00256-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HDAC1.
Protein Target Info & Infographic
Gene/Protein Information For HDAC1 (Source: Uniprot.org, NCBI)
Gene Name
HDAC1
Full Name
Histone deacetylase 1
Weight
55.103kDa
Superfamily
histone deacetylase family
Alternative Names
EC 3.5.1.98; GON-10; HD1; HD1DKFZp686H12203; Histone Deacetylase 1; KDAC1; reduced potassium dependency, yeast homolog-like 1; RPD3L1; RPD3L1RPD3 HDAC1 GON-10, HD1, KDAC1, RPD3, RPD3L1 histone deacetylase 1 histone deacetylase 1|reduced potassium dependency, yeast homolog-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HDAC1, check out the HDAC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HDAC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HDAC1 Mouse Monoclonal Antibody [Clone ID: OTI5F9] (M00256-1)
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