Data

Array ( [0] => store_id [1] => entity_id [2] => attribute_set_id [3] => type_id [4] => sku [5] => has_options [6] => required_options [7] => created_at [8] => updated_at [9] => mst_search_weight [10] => status [11] => name [12] => cj_search_index [13] => publication_count [14] => qty_in_warehouses [15] => is_recurring [16] => visibility [17] => tax_class_id [18] => conjugate [19] => featured [20] => featured_image [21] => fb_product [22] => custom_product_page [23] => free_shipping [24] => template [25] => host [26] => quantity_and_stock_status [27] => googleshopping_exclude [28] => yoast_keyword_score [29] => yoast_content_score [30] => price [31] => weight [32] => meta_title [33] => meta_description [34] => image [35] => small_image [36] => thumbnail [37] => url_key [38] => url_path [39] => options_container [40] => image_label [41] => small_image_label [42] => thumbnail_label [43] => msrp_display_actual_price_type [44] => gift_message_available [45] => size [46] => protein_name [47] => gene_full_name [48] => uniprot_id [49] => sensitivity [50] => applications [51] => reactivity [52] => form [53] => concentration [54] => cross_reactivity [55] => clone_number [56] => predicted_reactivity [57] => source_company [58] => isotype [59] => molecular_weight [60] => sample_size_available [61] => related_product_skus [62] => product_category [63] => research_category [64] => clonality [65] => swatch_image [66] => rating_value [67] => review_count [68] => mp_exclude_sitemap [69] => yoast_facebook_image [70] => yoast_twitter_image [71] => cj_related_products [72] => description [73] => short_description [74] => meta_keyword [75] => contents [76] => background [77] => gene_name [78] => synonyms [79] => application_details [80] => subcellular_localization [81] => tissue_specificity [82] => protein_function [83] => application_notes [84] => description_before_attributes [85] => competitor_equivalent_skus [86] => immunogen [87] => purification [88] => storage [89] => custom_attribute_1 [90] => options [91] => media_gallery [92] => extension_attributes [93] => tier_price [94] => tier_price_changed [95] => category_ids [96] => is_salable [97] => website_ids [98] => request_path [99] => rating_summary [100] => _cache_instance_store_filter [101] => media_gallery_images )
Key=store_id
Key=entity_id, value=76854
Key=attribute_set_id, value=4
Key=type_id, value=simple
Key=sku, value=A00227-Biotin
Key=has_options, value=1
Key=required_options, value=1
Key=created_at, value=2020-03-11 06:18:51
Key=updated_at, value=2024-09-26 01:03:56
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Anti-GAPDH Antibody (biotin)
Key=cj_search_index
Key=publication_count, value=0
Key=qty_in_warehouses
Key=is_recurring, value=No
Key=visibility, value=Catalog, Search
Key=tax_class_id, value=Taxable Goods
Key=conjugate
Key=featured, value=No
Key=featured_image, value=Yes
Key=fb_product, value=
Key=custom_product_page, value=No
Key=free_shipping, value=No
Key=template, value=antibodies
Key=host, value=Rabbit
Key=quantity_and_stock_status
Key=googleshopping_exclude, value=No
Key=yoast_keyword_score, value=-1368
Key=yoast_content_score, value=60
Key=price, value=449.0000
Key=weight, value=1.0000
Key=meta_title, value=Anti-GAPDH Antibody (biotin)
Key=meta_description, value=Boster Bio Anti-GAPDH Antibody (biotin) (Catalog # A00227-Biotin). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Rabbit, Chicken. Supplied as 0.1 mg in Liquid form antibody.
Key=image, value=/1/0/109-401-311-rabbit-a-il-8-1-wb-4x3.jpg
Key=small_image, value=/1/0/109-401-311-rabbit-a-il-8-1-wb-4x3.jpg
Key=thumbnail, value=/1/0/109-401-311-rabbit-a-il-8-1-wb-4x3.jpg
Key=url_key, value=anti-gapdh-antibody-biotin-a00227-biotin-boster
Key=url_path, value=anti-il-8-antibody-a00423-boster
Key=options_container, value=Block after Info Column
Key=image_label, value=Western blot analysis of IL-8 expression in Recombinant Human IL-8 50ng (lane 1). IL-8 at 11KD was detected using rabbit anti-IL-8 Antigen Affinity purified polyclonal antibody (Catalog # A00423) at 1:1000. The blot was developed using chemiluminescence (
Key=small_image_label, value=Western blot analysis of IL-8 expression in Recombinant Human IL-8 50ng (lane 1). IL-8 at 11KD was detected using rabbit anti-IL-8 Antigen Affinity purified polyclonal antibody (Catalog # A00423) at 1:1000. The blot was developed using chemiluminescence (
Key=thumbnail_label, value=Western blot analysis of IL-8 expression in Recombinant Human IL-8 50ng (lane 1). IL-8 at 11KD was detected using rabbit anti-IL-8 Antigen Affinity purified polyclonal antibody (Catalog # A00423) at 1:1000. The blot was developed using chemiluminescence (
Key=msrp_display_actual_price_type, value=Use config
Key=gift_message_available, value=No
Key=size, value=0.1 mg
Key=protein_name, value=Glyceraldehyde-3-phosphate dehydrogenase
Key=gene_full_name, value=Glyceraldehyde-3-phosphate dehydrogenase
Key=uniprot_id, value=P04406
Key=sensitivity
Key=applications, value=ELISA, WB
Key=reactivity, value=Chicken, Human, Mouse, Rabbit, Rat
Key=form, value=Liquid
Key=concentration, value=1 mg/mL
Key=cross_reactivity, value=Biotin-GAPDH antibody is human, mouse, rat, rabbit, and chicken reactive.
Key=clone_number
Key=predicted_reactivity, value=Bovine
Key=source_company, value=Prosci 3781-biotin; Cost: 249
Key=isotype, value=IgG
Key=molecular_weight, value=36053 MW
Key=sample_size_available, value=25ug for $99, contact us for details
Key=related_product_skus, value=EK0413,PB9621,PROTP10145,PROTP10145-1,PROTP10145-2,PROTP10145-3,PROTP19874,PROTP26894,PROTP41324,PROTP67813
Key=product_category, value=Primary Antibodies
Key=research_category, value=Neuroscience, Signal Transduction, Homeostasis
Key=clonality, value=Polyclonal
Key=swatch_image, value=/e/k/ek0852.png
Key=rating_value, value=100
Key=review_count, value=3
Key=mp_exclude_sitemap, value=No
Key=yoast_facebook_image, value=/m/0/m04858-lalba-primary-antibodies-if-testing-1.jpg
Key=yoast_twitter_image, value=/m/0/m04858-lalba-primary-antibodies-if-testing-1.jpg
Key=cj_related_products, value=Nuclear Receptors, Coactivators and Corepressors:A00227|A00227-1|H00227|M00227-6|A00227-HRP
Key=description, value=Boster Bio Anti-GAPDH Antibody (biotin) (Catalog # A00227-Biotin). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Rabbit, Chicken.
Key=short_description, value=Boster Bio Anti-GAPDH Antibody (biotin) (Catalog # A00227-Biotin). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Rabbit, Chicken.
Key=meta_keyword, value=Anti-GAPDH Antibody (biotin)
Key=contents, value=Biotin-GAPDH antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.
Key=background, value=Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Key=gene_name, value=GAPDH
Key=synonyms, value=Biotin-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD
Key=application_details, value=Biotin-GAPDH antibody can be used for detection of GAPDH by Western blot at 0.5 - 1 μ/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Key=subcellular_localization, value=Cytoplasm, cytosol
Key=tissue_specificity, value=Widely expressed, with the highest expression in lung, spleen and thymus.
Key=protein_function, value=Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Key=application_notes
Key=description_before_attributes
Key=competitor_equivalent_skus, value=sc 25342|sc 55462|sc 7165|sc 4600|sc 73321|sc 7922|sc 1269|sc 376750|sc 8427|sc 7302|sc 52870|sc 101499|sc 32817|sc 71367
Key=immunogen, value=Biotin-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.
Key=purification, value=Biotin-GAPDH antibody is affinity chromatography purified via peptide column.
Key=storage, value=Biotin-GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Key=custom_attribute_1, value=68 kDa
Key=options
Key=media_gallery
Key=extension_attributes
Key=tier_price
Key=tier_price_changed
Key=category_ids
Key=is_salable
Key=website_ids
Key=request_path
Key=rating_summary
Key=_cache_instance_store_filter
Key=media_gallery_images

Anti-GAPDH Antibody (biotin)

GAPDH antibody

Boster Bio Anti-GAPDH Antibody (biotin) (Catalog # A00227-Biotin). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Rabbit, Chicken.

Product Info Summary

SKU: A00227-Biotin
Size: 0.1 mg
Reactive Species: Chicken, Human, Mouse, Rabbit, Rat
Host: Rabbit
Application: ELISA, WB

Customers Who Bought This Also Bought

Product Name

Anti-GAPDH Antibody (biotin)

View all GAPDH Antibodies

SKU/Catalog Number

A00227-Biotin

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-GAPDH Antibody (biotin) (Catalog # A00227-Biotin). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Rabbit, Chicken.

Storage & Handling

Biotin-GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-GAPDH Antibody (biotin) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00227-Biotin)

Host

Rabbit

Contents

Biotin-GAPDH antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Biotin-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Biotin-GAPDH antibody is human, mouse, rat, rabbit, and chicken reactive.

Reactive Species

A00227-Biotin is reactive to GAPDH in Chicken, Human, Mouse, Rabbit, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

36053 MW

Background of GAPDH

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A00227-Biotin is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Biotin-GAPDH antibody can be used for detection of GAPDH by Western blot at 0.5 - 1 μ/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For GAPDH (Source: Uniprot.org, NCBI)

Gene Name

GAPDH

Full Name

Glyceraldehyde-3-phosphate dehydrogenase

Weight

36053 MW

Superfamily

glyceraldehyde-3-phosphate dehydrogenase family

Alternative Names

Biotin-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD GAPDH G3PD, GAPD, HEL-S-162eP glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase|OCAS, p38 component|Oct1 coactivator in S phase, 38 Kd component|aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GAPDH, check out the GAPDH Infographic

GAPDH infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GAPDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A00227-Biotin

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-GAPDH Antibody (biotin)?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-GAPDH Antibody (biotin)

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for Anti-GAPDH Antibody (biotin)

Order DetailsPrice
A00227-Biotin-0.1 mg

0.1 mg

$449

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
A00227-Biotin
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$449.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.