Anti-GAPDH Antibody (biotin)

GAPDH antibody

Boster Bio Anti-GAPDH Antibody (biotin) (Catalog # A00227-Biotin). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Rabbit, Chicken.

Product Info Summary

SKU: A00227-Biotin
Size: 0.1 mg
Reactive Species: Chicken, Human, Mouse, Rabbit, Rat
Host: Rabbit
Application: ELISA, WB

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Product Name

Anti-GAPDH Antibody (biotin)

View all GAPDH Antibodies

SKU/Catalog Number

A00227-Biotin

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-GAPDH Antibody (biotin) (Catalog # A00227-Biotin). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Rabbit, Chicken.

Storage & Handling

Biotin-GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-GAPDH Antibody (biotin) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00227-Biotin)

Host

Rabbit

Contents

Biotin-GAPDH antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Biotin-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Biotin-GAPDH antibody is human, mouse, rat, rabbit, and chicken reactive.

Reactive Species

A00227-Biotin is reactive to GAPDH in Chicken, Human, Mouse, Rabbit, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

36053 MW

Background of GAPDH

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00227-Biotin is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Biotin-GAPDH antibody can be used for detection of GAPDH by Western blot at 0.5 - 1 μ/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For GAPDH (Source: Uniprot.org, NCBI)

Gene Name

GAPDH

Full Name

Glyceraldehyde-3-phosphate dehydrogenase

Weight

36053 MW

Superfamily

glyceraldehyde-3-phosphate dehydrogenase family

Alternative Names

Biotin-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD GAPDH G3PD, GAPD, HEL-S-162eP glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase|OCAS, p38 component|Oct1 coactivator in S phase, 38 Kd component|aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GAPDH, check out the GAPDH Infographic

GAPDH infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GAPDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A00227-Biotin-0.1 mg

0.1 mg

$449

Free Secondary Antibody

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A00227-Biotin
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$449.00

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