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Product Info Summary
| SKU: | PA1067 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Phosphomevalonate kinase PMVK Antibody Picoband®
View all PMVK/phosphomevalonate kinase Antibodies
SKU/Catalog Number
PA1067
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Phosphomevalonate kinase PMVK Antibody catalog # PA1067. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phosphomevalonate kinase PMVK Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1067)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human PMVK, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1067 is reactive to PMVK in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
77 kDa
Calculated molecular weight
21995 MW
Background of PMVK/phosphomevalonate kinase
PMVK (phosphomevalonate kinase) is a cytosolic enzyme that catalyzes the conversion of mevalonate-5-phosphate to mevalonate-5-diphosphate. It is mapped to chromosome 1p13-1q22-23 and spans more than 8.4 kb in the human genome. PMVK is a peroxisomal protein which requires the C-terminal peroxisomal targeting signal, Ser-Arg-Leu, for localization to the organelle. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. And PMKase gene expression is subject to regulation by sterol at the level of transcription. It is a single copy gene covering less than 15 kb in the human genome. The human PMKase amino acid sequence contains a consensus peroxisomal targeting sequence (PTS-1), Ser-Arg-Leu, at the C terminus of the protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1067 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse
Positive Control
WB: Rat Cardiac Muscle Tissue
IHC: Rat Skeletal Muscle Tissue
Validation Images & Assay Conditions
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Anti-PMVK antibody, PA1067, Western blotting
WB: Rat Cardiac Muscle Tissue Lysate
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Anti-PMVK antibody, PA1067, IHC(P)
IHC(P): Rat Skeletal Muscle Tissue
Protein Target Info & Infographic
Gene/Protein Information For PMVK (Source: Uniprot.org, NCBI)
Gene Name
PMVK
Full Name
Phosphomevalonate kinase
Weight
21995 MW
Alternative Names
Phosphomevalonate kinase;PMKase;hPMK;2.7.4.2;PMVK;PMKI; PMVK HUMPMKI, PMK, PMKA, PMKASE, POROK1 phosphomevalonate kinase phosphomevalonate kinase|testis tissue sperm-binding protein Li 95mP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PMVK, check out the PMVK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PMVK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phosphomevalonate kinase PMVK Antibody Picoband® (PA1067)
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Customer Q&As
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4 Customer Q&As for Anti-Phosphomevalonate kinase PMVK Antibody Picoband®
Question
Can you help my question with product PA1067, anti-PMVK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1067 anti-PMVK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-31
Question
We are currently using anti-PMVK antibody PA1067 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
The anti-PMVK antibody (PA1067) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-16
Question
Would PA1067 anti-PMVK antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
As indicated on the product datasheet, PA1067 anti-PMVK antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-28
Question
I see that the anti-PMVK antibody PA1067 works with WB, what is the protocol used to produce the result images on the product page?
M. Miller
Verified customer
Asked: 2015-06-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-06-23
