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Product Info Summary
| SKU: | A00470 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Galectin 1/Lgals1 Antibody Picoband®
View all Galectin-1 Antibodies
SKU/Catalog Number
A00470
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Galectin 1/Lgals1 Antibody Picoband® catalog # A00470. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Galectin 1/Lgals1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00470)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Galectin 1 recombinant protein (Position: A2-E135).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00470 is reactive to Lgals1 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
15 kDa
Calculated molecular weight
33477 MW
Background of Galectin-1
Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. It is thought to play a role in creating immune tolerance in pregnancy. It has been found that Galectin-1-mediated production of IL6 may assist in augmenting the innate immune response against NiV. Galectin-1 may also be a significant factor that augments the efficiency of the HIV-1 infection process.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00470 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WB: mouse thymus tissue, mouse kidney tissue, mouse testis tissue, mouse ovary tissue, rat heart tissue, rat skeletal muscle tissue, rat gaster tissue, rat testis tissue
IHC: mouse kidney tissue, rat kidney tissue, rat small intestine tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Galectin 1 using anti-Galectin 1 antibody (A00470).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse thymus tissue lysates,
Lane 2: mouse kidney tissue lysates,
Lane 3: mouse testis tissue lysates,
Lane 4: mouse ovary tissue lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat skeletal muscle tissue lysates,
Lane 7: rat gaster tissue lysates,
Lane 8: rat testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 1 antigen affinity purified polyclonal antibody (Catalog # A00470) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 1 at approximately 15KD. The expected band size for Galectin 1 is at 15KD.
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Figure 2. IHC analysis of Galectin 1 using anti-Galectin 1 antibody (A00470).
Galectin 1 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Galectin 1 Antibody (A00470) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of Galectin 1 using anti-Galectin 1 antibody (A00470).
Galectin 1 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Galectin 1 Antibody (A00470) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Galectin 1 using anti-Galectin 1 antibody (A00470).
Galectin 1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Galectin 1 Antibody (A00470) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 5. Sandwich ELISA - Recombinant mouse Galectin 1/Lgals1 protein standard curve.
Use in combination with reagents from Mouse Galectin 1/Lgals1 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0763).
Protein Target Info & Infographic
Gene/Protein Information For Lgals1 (Source: Uniprot.org, NCBI)
Gene Name
Lgals1
Full Name
Galectin-1
Weight
33477 MW
Alternative Names
Galectin-1; Gal-1; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; S-Lac lectin 1; Lgals1; Gbp; LGALS1 GAL1, GBP galectin 1 galectin-1|14 kDa laminin-binding protein|14 kDa lectin|HBL|HLBP14|HPL|S-Lac lectin 1|beta-galactoside-binding lectin L-14-I|beta-galactoside-binding protein 14kDa|epididymis secretory sperm binding protein|gal-1|galaptin|lactose-binding lectin 1|lectin, galactoside-binding, soluble, 1|putative MAPK-activating protein PM12
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Lgals1, check out the Lgals1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Lgals1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Galectin 1/Lgals1 Antibody Picoband® (A00470)
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Customer Q&As
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4 Customer Q&As for Anti-Galectin 1/Lgals1 Antibody Picoband®
Question
My colleagues were content with the WB result of your anti-Galectin 1/Lgals1 antibody. However we have been able to see positive staining in placenta skin extracellular space using this antibody. Is that expected? Could you tell me where is LGALS1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-04-12
Answer
From literature, placenta skin does express LGALS1. Generally LGALS1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have LGALS1 expression, here are a few articles citing expression in various tissues:
Hepatoma, Pubmed ID: 2719646
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 2719964
Lung fibroblast, Pubmed ID: 12761501
Lymphocyte, Pubmed ID: 1988031
Mammary cancer, Pubmed ID: 19497882
Melanoma, Pubmed ID: 1386213
Placenta, Pubmed ID: 3065332, 3611046, 10369126, 18824694
Placenta, and Promyelocytic leukemia, Pubmed ID: 2910856
Placenta, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Spleen, Pubmed ID: 2383549
Thalamus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-04-12
Question
We ordered your anti-Galectin 1/Lgals1 antibody for WB on liver in a previous project. I am using mouse, and We are going to use the antibody for IHC next. I am interested in examining liver as well as spleen in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2017-10-30
Answer
I have checked the website and datasheets of our anti-Galectin 1/Lgals1 antibody and I see that A00470 has been validated on mouse in both WB and IHC. Thus A00470 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-10-30
Question
We have seen staining in rat lung fibroblast. Any tips? Is anti-Galectin 1/Lgals1 antibody supposed to stain lung fibroblast positively?
A. Bhatt
Verified customer
Asked: 2015-12-11
Answer
Based on literature lung fibroblast does express LGALS1. Based on Uniprot.org, LGALS1 is expressed in adipose tissue, lung, placenta promyelocytic leukemia, hepatoma, placenta, lymphocyte, lung fibroblast, thalamus, placenta skin, brain, platelet, brain, cajal-retzius cell fetal brain cortex, melanoma, spleen, mammary cancer, liver, among other tissues. Regarding which tissues have LGALS1 expression, here are a few articles citing expression in various tissues:
Hepatoma, Pubmed ID: 2719646
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 2719964
Lung fibroblast, Pubmed ID: 12761501
Lymphocyte, Pubmed ID: 1988031
Mammary cancer, Pubmed ID: 19497882
Melanoma, Pubmed ID: 1386213
Placenta, Pubmed ID: 3065332, 3611046, 10369126, 18824694
Placenta, and Promyelocytic leukemia, Pubmed ID: 2910856
Placenta, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Spleen, Pubmed ID: 2383549
Thalamus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-12-11
Question
We are currently using anti-Galectin 1/Lgals1 antibody A00470 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on dog tissues as well?
D. Singh
Verified customer
Asked: 2015-04-01
Answer
The anti-Galectin 1/Lgals1 antibody (A00470) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-01
