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Product Info Summary
| SKU: | A04246 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Cyclin-G-associated kinase GAK Antibody
SKU/Catalog Number
A04246
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Cyclin-G-associated kinase GAK Antibody (Catalog # A04246). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin-G-associated kinase GAK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04246)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Internal of human GAK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04246 is reactive to GAK in Human, Mouse
Reconstitution
Calculated molecular weight
143191 MW
Background of GAK
Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.
Kimura S.H., Genomics 44:179-187(1997).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Greener T., J. Biol. Chem. 275:1365-1370(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04246 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from 293 cells, using GAK antibody A04246.
Protein Target Info & Infographic
Gene/Protein Information For GAK (Source: Uniprot.org, NCBI)
Gene Name
GAK
Full Name
Cyclin-G-associated kinase
Weight
143191 MW
Superfamily
protein kinase superfamily
Alternative Names
Cyclin-G-associated kinase;2.7.11.1;GAK; GAK DNAJ26, DNAJC26 cyclin G associated kinase cyclin-G-associated kinase|auxilin-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GAK, check out the GAK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GAK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin-G-associated kinase GAK Antibody (A04246)
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No publications found for A04246
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Customer Q&As
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13 Customer Q&As for Anti-Cyclin-G-associated kinase GAK Antibody
Question
Will anti-GAK antibody A04246 work for WB with fibroblast?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
According to the expression profile of fibroblast, GAK is highly expressed in fibroblast. So, it is likely that anti-GAK antibody A04246 will work for WB with fibroblast.
Boster Scientific Support
Answered: 2020-05-08
Question
My question regarding product A04246, anti-GAK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04246 anti-GAK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-23
Question
Does anti-GAK antibody A04246 work on horse WB with endothelial cell?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
Our lab technicians have not tested anti-GAK antibody A04246 on horse. You can run a BLAST between horse and the immunogen sequence of anti-GAK antibody A04246 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse endothelial cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-02
Question
I would like to test anti-GAK antibody A04246 on human fibroblast for research purposes, then I may be interested in using anti-GAK antibody A04246 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
The products we sell, including anti-GAK antibody A04246, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-04
Question
We are currently using anti-GAK antibody A04246 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
The anti-GAK antibody (A04246) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-08
Question
Is a blocking peptide available for product anti-GAK antibody (A04246)?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
We do provide the blocking peptide for product anti-GAK antibody (A04246). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-30
Question
Is there a BSA free version of anti-GAK antibody A04246 available?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GAK antibody A04246 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-19
Question
Would A04246 anti-GAK antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
It shows on the product datasheet, A04246 anti-GAK antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-22
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fibroblast using anti-GAK antibody A04246. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-19
Question
Here is the WB image, lot number and protocol we used for fibroblast using anti-GAK antibody A04246. Please let me know if you require anything else.
O. Krishna
Verified customer
Asked: 2019-08-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-05
Question
I see that the anti-GAK antibody A04246 works with WB, what is the protocol used to produce the result images on the product page?
D. Jones
Verified customer
Asked: 2019-07-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-10
Question
I was wanting to use your anti-GAK antibody for WB for human fibroblast on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fibroblast identification?
Verified Customer
Verified customer
Asked: 2018-03-28
Answer
You can see on the product datasheet, A04246 anti-GAK antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human fibroblast in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-28
Question
Is this A04246 anti-GAK antibody reactive to the isotypes of GAK?
R. Banerjee
Verified customer
Asked: 2017-01-23
Answer
The immunogen of A04246 anti-GAK antibody is Synthesized peptide derived from Internal of human GAK. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-01-23
