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Product Info Summary
| SKU: | A00728-3 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-Smad1 (Ab-465) Antibody
SKU/Catalog Number
A00728-3
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Smad1 (Ab-465) Antibody (Catalog # A00728-3). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Smad1 (Ab-465) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00728-3)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa. 461~465 (I-S-S-V-S) derived from Human Smad1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00728-3 is reactive to SMAD1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
52157 MW
Background of SMAD1
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD).
Hiroaki Seto, et al. (2004) J Clin Invest. March 1; 113(5): 718
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00728-3 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 55kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SMAD1 using anti-SMAD1 antibody (A00728-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD1 antigen affinity purified polyclonal antibody (Catalog # A00728-3) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for SMAD1.
Click image to see more details
Immunofluorescence staining of methanol-fixed Hela cells using Smad1(Ab-465) Antibody #A00728-3.
Protein Target Info & Infographic
Gene/Protein Information For SMAD1 (Source: Uniprot.org, NCBI)
Gene Name
SMAD1
Full Name
Mothers against decapentaplegic homolog 1
Weight
52157 MW
Superfamily
dwarfin/SMAD family
Alternative Names
Mothers against decapentaplegic homolog 1;MAD homolog 1;Mothers against DPP homolog 1;Dwarfin-A;Dwf-A;Mothers-against-DPP-related 1;Mad-related protein 1;mMad1;SMAD family member 1;SMAD 1;Smad1;Smad1;Madh1, Madr1; SMAD1 BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1 SMAD family member 1 mothers against decapentaplegic homolog 1|MAD, mothers against decapentaplegic homolog 1|Mad-related protein 1|SMAD, mothers against DPP homolog 1|TGF-beta signaling protein 1|mothers against DPP homolog 1|transforming growth factor-beta signaling protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMAD1, check out the SMAD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMAD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Smad1 (Ab-465) Antibody (A00728-3)
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No publications found for A00728-3
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Customer Q&As
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16 Customer Q&As for Anti-Smad1 (Ab-465) Antibody
Question
I would like to test anti-Smad1 (Ab-465) antibody A00728-3 on human placenta for research purposes, then I may be interested in using anti-Smad1 (Ab-465) antibody A00728-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
The products we sell, including anti-Smad1 (Ab-465) antibody A00728-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-07
Question
Our team were happy with the WB result of your anti-Smad1 (Ab-465) antibody. However we have been able to see positive staining in uterus cytoplasm. using this antibody. Is that expected? Could you tell me where is SMAD1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
Based on literature, uterus does express SMAD1. Generally SMAD1 expresses in cytoplasm. Regarding which tissues have SMAD1 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 8637600
Mammary carcinoma, Pubmed ID: 8663601
Ovarian carcinoma, Pubmed ID: 9136927
Placenta, Pubmed ID: 8774881, 15489334
Uterus, Pubmed ID: 14702039, 17974005
Boster Scientific Support
Answered: 2020-02-07
Question
We have observed staining in mouse mammary carcinoma. Are there any suggestions? Is anti-Smad1 (Ab-465) antibody supposed to stain mammary carcinoma positively?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
From literature mammary carcinoma does express SMAD1. From Uniprot.org, SMAD1 is expressed in visceral pleura, kidney, mammary carcinoma, placenta, uterus, ovarian carcinoma, among other tissues. Regarding which tissues have SMAD1 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 8637600
Mammary carcinoma, Pubmed ID: 8663601
Ovarian carcinoma, Pubmed ID: 9136927
Placenta, Pubmed ID: 8774881, 15489334
Uterus, Pubmed ID: 14702039, 17974005
Boster Scientific Support
Answered: 2020-01-24
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Smad1 (Ab-465) antibody A00728-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-20
Question
Will anti-Smad1 (Ab-465) antibody A00728-3 work for IF with placenta?
E. Roberts
Verified customer
Asked: 2019-12-27
Answer
According to the expression profile of placenta, SMAD1 is highly expressed in placenta. So, it is likely that anti-Smad1 (Ab-465) antibody A00728-3 will work for IF with placenta.
Boster Scientific Support
Answered: 2019-12-27
Question
Is this A00728-3 anti-Smad1 (Ab-465) antibody reactive to the isotypes of SMAD1?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
The immunogen of A00728-3 anti-Smad1 (Ab-465) antibody is Peptide sequence around aa. 461~465 (I-S-S-V-S) derived from Human Smad1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-31
Question
I have a question about product A00728-3, anti-Smad1 (Ab-465) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00728-3 anti-Smad1 (Ab-465) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-03
Question
Our lab used your anti-Smad1 (Ab-465) antibody for WB on mammary carcinoma a few months ago. I am using human, and We intend to use the antibody for IF next. I am looking for examining mammary carcinoma as well as kidney in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
I have checked the website and datasheets of our anti-Smad1 (Ab-465) antibody and it seems that A00728-3 has been tested on human in both WB and IF. Thus A00728-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-27
Question
We are currently using anti-Smad1 (Ab-465) antibody A00728-3 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
The anti-Smad1 (Ab-465) antibody (A00728-3) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-03
Question
Is a blocking peptide available for product anti-Smad1 (Ab-465) antibody (A00728-3)?
Verified Customer
Verified customer
Asked: 2018-12-03
Answer
We do provide the blocking peptide for product anti-Smad1 (Ab-465) antibody (A00728-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-12-03
Question
I see that the anti-Smad1 (Ab-465) antibody A00728-3 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-10-30
Question
I was wanting to use your anti-Smad1 (Ab-465) antibody for IF for human placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human placenta identification?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
As indicated on the product datasheet, A00728-3 anti-Smad1 (Ab-465) antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-03
Question
Will A00728-3 anti-Smad1 (Ab-465) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
W. Parker
Verified customer
Asked: 2015-02-26
Answer
As indicated on the product datasheet, A00728-3 anti-Smad1 (Ab-465) antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-02-26
Question
Do you have a BSA free version of anti-Smad1 (Ab-465) antibody A00728-3 available?
S. Parker
Verified customer
Asked: 2014-10-22
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Smad1 (Ab-465) antibody A00728-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-10-22
Question
We need using your anti-Smad1 (Ab-465) antibody for protein deubiquitination studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
J. Zhao
Verified customer
Asked: 2014-02-11
Answer
Thanks for your inquiry. This A00728-3 anti-Smad1 (Ab-465) antibody is validated on hela cells. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-02-11
Question
Please see the WB image, lot number and protocol we used for placenta using anti-Smad1 (Ab-465) antibody A00728-3. Please let me know if you require anything else.
R. Jackson
Verified customer
Asked: 2013-02-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-02-22
