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Product Info Summary
| SKU: | A03539-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-FRZB Antibody Picoband®
View all sFRP-3/FRZB Antibodies
SKU/Catalog Number
A03539-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FRZB Antibody Picoband® catalog # A03539-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FRZB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03539-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse FRZB recombinant protein (Position: N49-K311).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03539-2 is reactive to Frzb in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
75187 MW
Background of sFRP-3/FRZB
FRZB is also known as FRE or OS1. The protein encoded by this gene is a secreted protein that is involved in the regulation of bone development. Defects in this gene are a cause of female-specific osteoarthritis (OA) susceptibility. FRZB is a Wnt-binding protein especially important in embryonic development. It is a competitor for the cell-surface G-protein receptor Frizzled. FRZB is localized in the extracellular plasma membrane. Unlike frizzled, frzb lacks the 7 transmembrane domains normally found in G-protein-coupled receptors. It is still considered a homolog of frizzled because it contains a Cysteine Rich Domain (CRD), and because of its intracellular C-terminus which is crucial for signaling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03539-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat brain tissue, rat kidney tissue, rat heart tissue, mouse brain tissue, mouse kidney tissue, mouse heart tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FRZB(SFRP3) using anti-FRZB(SFRP3) antibody (A03539-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse kidney tissue lysates,
Lane 6: mouse heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRZB(SFRP3) antigen affinity purified polyclonal antibody (Catalog # A03539-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRZB(SFRP3) at approximately 36KD. The expected band size for FRZB(SFRP3) is at 36KD.
Protein Target Info & Infographic
Gene/Protein Information For Frzb (Source: Uniprot.org, NCBI)
Gene Name
Frzb
Full Name
Secreted frizzled-related protein 3
Weight
75187 MW
Superfamily
secreted frizzled-related protein (sFRP) family
Alternative Names
Secreted frizzled-related protein 3; sFRP-3; Frezzled; Fritz; Frizzled-related protein 1; FrzB-1; Frzb; Fiz; Fre; Frzb1; Sfrp3 FRZB FRE, FRITZ, FRP-3-1, FRZB-PEN, FRZB1, FZRB, OS1, SFRP3, SRFP3, hFIZ, FRZB frizzled related protein secreted frizzled-related protein 3|frezzled|frizzled homolog-related|frizzled-related protein 1|sFRP-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Frzb, check out the Frzb Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Frzb: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FRZB Antibody Picoband® (A03539-2)
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