Anti-FRZB Antibody Picoband®

Product Name

Anti-FRZB Antibody Picoband®

View all sFRP-3/FRZB Antibodies

SKU/Catalog Number

PB9372

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FRZB Antibody Picoband® catalog # PB9372. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FRZB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9372)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human FRZB recombinant protein (Position: E176-N325). Human FRZB shares 90% amino acid (aa) sequence identity with mouse FRZB.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9372 is reactive to FRZB in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

36 kDa

Calculated molecular weight

36254 MW

Background of sFRP-3/FRZB

FRZB is also known as FRE or OS1. The protein encoded by this gene is a secreted protein that is involved in the regulation of bone development. Defects in this gene are a cause of female-specific osteoarthritis (OA) susceptibility. FRZB is a Wnt-binding protein especially important in embryonic development. It is a competitor for the cell-surface G-protein receptor Frizzled. FRZB is localized in the extracellular plasma membrane. Unlike frizzled, frzb lacks the 7 transmembrane domains normally found in G-protein-coupled receptors. It is still considered a homolog of frizzled because it contains a Cysteine Rich Domain (CRD), and because of its intracellular C-terminus which is crucial for signaling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9372 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, -
ELISA, 0.1-0.5μg/ml, -

Positive Control

WB: PANC Whole Cell, U87 Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For FRZB (Source: Uniprot.org, NCBI)

Alternative Names

Secreted frizzled-related protein 3;sFRP-3;Frezzled;Fritz;Frizzled-related protein 1;FrzB-1;FRZB;FIZ, FRE, FRP, FRZB1, SFRP3; FRZB FRE, FRITZ, FRP-3-1, FRZB-PEN, FRZB1, FZRB, OS1, SFRP3, SRFP3, hFIZ, FRZB frizzled related protein secreted frizzled-related protein 3|frezzled|frizzled homolog-related|frizzled-related protein 1|sFRP-3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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