Product Info Summary
SKU: | A02802-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-ADAMTS5 Antibody Picoband®
SKU/Catalog Number
A02802-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ADAMTS5 Antibody Picoband® catalog # A02802-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADAMTS5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02802-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ADAMTS5 recombinant protein (Position: D747-K780).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A02802-1 is reactive to ADAMTS5 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
43787 MW
Background of ADAMTS5
ADAMTS5 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 5), is an enzyme that in humans is encoded by the ADAMTS5 gene. ADAMTS5 is a member of the large ADAMTS family of zinc-dependent proteases. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. By somatic cell hybrid analysis, the human ADAMTS5 gene is mapped to chromosome 21. Used mouse models, it is showed that Sdc4 controls a pathway that activates Adamts5 at the chondrocyte cell surface through Erk1/Erk2 activation of Mmp3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02802-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
ELISA, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human U20S whole cell, human SW620 whole cell, human THP-1 whole cell, human Raji whole cell, human SGC-7901 whole cell, rat liver tissue, rat testis tissue, mouse liver tissue, mouse testis tissue,
IHC: human placenta tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ADAMTS5 using anti-ADAMTS5 antibody (A02802-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: human SW620 whole cell lysates,
Lane 5: human THP-1 whole cell lysates,
Lane 6: human Raji whole cell lysates,
Lane 7: human SGC-7901 whole cell lysates,
Lane 8: rat liver tissue lysates,
Lane 9: rat testis tissue lysates,
Lane 10: mouse liver tissue lysates,
Lane 11: mouse testis tissue lysates,
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAMTS5 antigen affinity purified polyclonal antibody (Catalog # A02802-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAMTS5 at approximately 75 kDa. The expected band size for ADAMTS5 is at 102 kDa.
Click image to see more details
Figure 2. IHC analysis of ADAMTS5 using anti-ADAMTS5 antibody (A02802-1).
ADAMTS5 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADAMTS5 Antibody (A02802-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ADAMTS5 (Source: Uniprot.org, NCBI)
Gene Name
ADAMTS5
Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 5
Weight
43787 MW
Alternative Names
Proliferation-associated protein 2G4;Cell cycle protein p38-2G4 homolog;hG4-1;ErbB3-binding protein 1;PA2G4;EBP1; ADAMTS5 ADAM-TS 11, ADAM-TS 5, ADAM-TS5, ADAMTS-11, ADAMTS-5, ADAMTS11, ADMP-2 ADAM metallopeptidase with thrombospondin type 1 motif 5 A disintegrin and metalloproteinase with thrombospondin motifs 5|a disintegrin and metalloproteinase with thrombospondin motifs 11|a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2)|aggrecanase-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADAMTS5, check out the ADAMTS5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADAMTS5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADAMTS5 Antibody Picoband® (A02802-1)
Hello CJ!
A02802-1 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Physalin A Inhibits MAPK and NF-κB Signal Transduction Through Integrin αVβ3 and Exerts Chondroprotective Effect.
Selective STAT3 Inhibitor Alantolactone Ameliorates Osteoarthritis via Regulating Chondrocyte Autophagy and Cartilage Homeostasis
Protease-activated receptor 2 (PAR-2) antagonist AZ3451 as a novel therapeutic agent for osteoarthritis
MCC950, the NLRP3 Inhibitor, Protects against Cartilage Degradation in a Mouse Model of Osteoarthritis
p-Coumaric Acid Attenuates IL-1β-Induced Inflammatory Responses and Cellular Senescence in Rat Chondrocytes
Huang X,Pei W,Ni B,Zhang R,You H.Chondroprotective and antiarthritic effects of Galangin in Osteoarthritis: An in Vitro and in Vivo Study.Eur J Pharmacol.2021 Jun 3:174232.doi:10.1016/j. ejphar.2021. 174232.Epub ahead of print.PMID:34090897.
Species: Rat
A02802-1 usage in article: APP:WB, SAMPLE :CHONDROCYTES, DILUTION:NA
Chu X,Yu T,Huang X,Xi Y,Ni B,Zhang R,You H.Tomatidine suppresses inflammation in primary articular chondrocytes and attenuates cartilage degradation in osteoarthritic rats.Aging (Albany NY).2020 Jul 5;12(13):12799-12811.doi:10.18632/aging.103222.Epub 2020
Species: Rat
A02802-1 usage in article: APP:WB, SAMPLE:PRIMARY CHONDROCYTES, DILUTION:1:1000
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