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Product Info Summary
| SKU: | PB9194 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-FOXM1 Antibody Picoband®
SKU/Catalog Number
PB9194
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FOXM1 Antibody Picoband® catalog # PB9194. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FOXM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9194)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FOXM1 recombinant protein (Position: A411-Q763). Human FOXM1 shares 75% and 76% amino acid (aa) sequences identity with mouse and rat FOXM1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9194 is reactive to FOXM1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
84283 MW
Background of FOXM1
Forkhead box protein M1, also called FOXM1, is a protein that in humans is encoded by the FOXM1 gene. It is mapped to 12p13.33. The protein encoded by this gene is a member of the FOX family of transcription factors. FOXM1 is known to play a key role in cell cycle progression where endogenous FOXM1 expression peaks at S and G2/M phases and also in the control of cell proliferation. FOXM1 gene is now known as a human proto-oncogene. Abnormal upregulation of FOXM1 is involved in the oncogenesis of basal cell carcinoma, the most common human cancer worldwide. It was hypothesized that FOXM1 induces cancer initiation through stem/progenitor cell expansion. What's more, FOXM1 has been shown to modulate the epigenome. It was found that overexpression of FOXM1 "brain washes" normal cells to adopt cancer-like epigenome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9194 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human COLO320 whole cell, human SW620 whole cell, human SKOV whole cell, human MCD-7 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FOXM1 using anti-FOXM1 antibody (PB9194).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human COLO320 whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: human SKOV whole cell lysates,
Lane 5: human MCD-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXM1 antigen affinity purified polyclonal antibody (Catalog # PB9194) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXM1 at approximately 84 kDa. The expected band size for FOXM1 is at 84 kDa.
Click image to see more details
Figure 2. Western blot analysis of FOXM1 using anti-FOXM1 antibody (PB9194).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human FOXM1 protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXM1 antigen affinity purified polyclonal antibody (Catalog # PB9194) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXM1 at approximately 36 kDa. The expected band size for FOXM1 is at 36 kDa.
Protein Target Info & Infographic
Gene/Protein Information For FOXM1 (Source: Uniprot.org, NCBI)
Gene Name
FOXM1
Full Name
Forkhead box protein M1
Weight
84283 MW
Alternative Names
Forkhead box protein M1;Forkhead-related protein FKHL16;Hepatocyte nuclear factor 3 forkhead homolog 11;HFH-11;HNF-3/fork-head homolog 11;M-phase phosphoprotein 2;MPM-2 reactive phosphoprotein 2;Transcription factor Trident;Winged-helix factor from INS-1 cells;FOXM1;FKHL16, HFH11, MPP2, WIN; FOXM1 FKHL16A, FOXM1B, FOXM1C, HFH-11, HFH11, HNF-3, INS-1, MPHOSPH2, MPP-2, MPP2, PIG29, TRIDENT, FOXM1 forkhead box M1 forkhead box protein M1|Forkhead, drosophila, homolog-like 16|HNF-3/fork-head homolog 11|M-phase phosphoprotein 2|MPM-2 reactive phosphoprotein 2|forkhead box M1-D|forkhead-related protein FKHL16|hepatocyte nuclear factor 3 forkhead homolog 11|transcription factor Trident|winged-helix factor from INS-1 cells
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXM1, check out the FOXM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FOXM1 Antibody Picoband® (PB9194)
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Customer Q&As
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15 Customer Q&As for Anti-FOXM1 Antibody Picoband®
Question
Our lab want to know about using your anti-FOXM1 antibody for negative regulation of transcription studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
We appreciate your inquiry. This PB9194 anti-FOXM1 antibody is validated on hela whole cell lysate, colo320 whole cell lysate, sw620 whole cell lysate, skov whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-26
Question
I was wanting to use your anti-FOXM1 antibody for WB for human lymphoblast on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lymphoblast identification?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
As indicated on the product datasheet, PB9194 anti-FOXM1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymphoblast in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-14
Question
We are currently using anti-FOXM1 antibody PB9194 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The anti-FOXM1 antibody (PB9194) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-27
Question
We have seen staining in human ovary skin. What should we do? Is anti-FOXM1 antibody supposed to stain ovary skin positively?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
Based on literature ovary skin does express FOXM1. Based on Uniprot.org, FOXM1 is expressed in endometrium epithelium, colon carcinoma, pancreatic carcinoma testis, lung, ovary skin, lymphoblast, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have FOXM1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 9032290
Lung, Ovary, and Skin, Pubmed ID: 15489334
Lymphoblast, Pubmed ID: 8290587
Pancreatic carcinoma, and Testis, Pubmed ID: 9242644
Boster Scientific Support
Answered: 2020-01-21
Question
Is this PB9194 anti-FOXM1 antibody reactive to the isotypes of FOXM1?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
The immunogen of PB9194 anti-FOXM1 antibody is E.coli-derived human FOXM1 recombinant protein (Position: A411-Q763). Human FOXM1 shares 75% and 76% amino acid (aa) sequences identity with mouse and rat FOXM1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-11
Question
Does anti-FOXM1 antibody PB9194 work for WB with lymphoblast?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
According to the expression profile of lymphoblast, FOXM1 is highly expressed in lymphoblast. So, it is likely that anti-FOXM1 antibody PB9194 will work for WB with lymphoblast.
Boster Scientific Support
Answered: 2019-12-09
Question
Is there a BSA free version of anti-FOXM1 antibody PB9194 available?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FOXM1 antibody PB9194 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-25
Question
Is a blocking peptide available for product anti-FOXM1 antibody (PB9194)?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
We do provide the blocking peptide for product anti-FOXM1 antibody (PB9194). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-25
Question
My boss were content with the WB result of your anti-FOXM1 antibody. However we have seen positive staining in cervix carcinoma erythroleukemia nucleus. using this antibody. Is that expected? Could you tell me where is FOXM1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
From literature, cervix carcinoma erythroleukemia does express FOXM1. Generally FOXM1 expresses in nucleus. Regarding which tissues have FOXM1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 9032290
Lung, Ovary, and Skin, Pubmed ID: 15489334
Lymphoblast, Pubmed ID: 8290587
Pancreatic carcinoma, and Testis, Pubmed ID: 9242644
Boster Scientific Support
Answered: 2019-09-18
Question
I see that the anti-FOXM1 antibody PB9194 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-12
Question
See below the WB image, lot number and protocol we used for lymphoblast using anti-FOXM1 antibody PB9194. Please let me know if you require anything else.
C. Parker
Verified customer
Asked: 2019-04-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-30
Question
We need to test anti-FOXM1 antibody PB9194 on human lymphoblast for research purposes, then I may be interested in using anti-FOXM1 antibody PB9194 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Gonzalez
Verified customer
Asked: 2019-03-05
Answer
The products we sell, including anti-FOXM1 antibody PB9194, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-05
Question
Can you help my question with product PB9194, anti-FOXM1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-28
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9194 anti-FOXM1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-28
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymphoblast using anti-FOXM1 antibody PB9194. Let me know if you need anything else.
B. Walker
Verified customer
Asked: 2017-03-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-03-23
Question
Does PB9194 anti-FOXM1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Wu
Verified customer
Asked: 2013-07-23
Answer
You can see on the product datasheet, PB9194 anti-FOXM1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-07-23
