Click image to see more details
Product Info Summary
| SKU: | A02807-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PIAS3 Antibody Picoband®
SKU/Catalog Number
A02807-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PIAS3 Antibody Picoband® catalog # A02807-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PIAS3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02807-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human PIAS3, which shares 97.6% amino acid (aa) sequence identity with both mouse and rat PIAS3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02807-1 is reactive to PIAS3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
68 kDa
Calculated molecular weight
96950 MW
Background of PIAS3
E3 SUMO-protein ligase PIAS3 is an enzyme that in humans is encoded by the PIAS3 gene. This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02807-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: human Hela cell, human U-87MG cell, human COLO-320 cell, human HepG2 cell, rat brain tissue, mouse NIH3T3 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PIAS3 using anti-PIAS3 antibody (A02807-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela cell lysate,
Lane 2: human U-87MG cell lysate,
Lane 3: human COLO-320 cell lysate,
Lane 4: human HepG2 cell lysate,
Lane 5: rat brain tissue lysate,
Lane 6: mouse NIH3T3 cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIAS3 antigen affinity purified polyclonal antibody (Catalog # A02807-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIAS3 at approximately 68KD. The expected band size for PIAS3 is at 68KD.
Protein Target Info & Infographic
Gene/Protein Information For PIAS3 (Source: Uniprot.org, NCBI)
Gene Name
PIAS3
Full Name
E3 SUMO-protein ligase PIAS3
Weight
96950 MW
Superfamily
PIAS family
Alternative Names
E3 SUMO-protein ligase PIAS3; Protein inhibitor of activated STAT protein 3; PIAS3 PIAS3 ZMIZ5 protein inhibitor of activated STAT 3 E3 SUMO-protein ligase PIAS3|E3 SUMO-protein transferase PIAS3|protein inhibitor of activated STAT protein 3|zinc finger, MIZ-type containing 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PIAS3, check out the PIAS3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PIAS3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PIAS3 Antibody Picoband® (A02807-1)
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Customer Q&As
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4 Customer Q&As for Anti-PIAS3 Antibody Picoband®
Question
Please see the WB image, lot number and protocol we used for mammary cancer using anti-PIAS3 antibody A02807-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-04
Question
Does anti-PIAS3 antibody A02807-1 work on goat WB with mammary cancer?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
Our lab technicians have not tested anti-PIAS3 antibody A02807-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-PIAS3 antibody A02807-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat mammary cancer in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-05
Question
We are currently using anti-PIAS3 antibody A02807-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
The anti-PIAS3 antibody (A02807-1) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-31
Question
Would anti-PIAS3 antibody A02807-1 work for WB with mammary cancer?
K. Taylor
Verified customer
Asked: 2015-01-26
Answer
According to the expression profile of mammary cancer, PIAS3 is highly expressed in mammary cancer. So, it is likely that anti-PIAS3 antibody A02807-1 will work for WB with mammary cancer.
Boster Scientific Support
Answered: 2015-01-26
