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Product Info Summary
| SKU: | A01295 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-FLIP CFLAR Antibody
SKU/Catalog Number
A01295
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-FLIP CFLAR Antibody (Catalog # A01295). Tested in ELISA, WB, ICC applications. This antibody reacts with Mouse.
Storage & Handling
FLIP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-FLIP CFLAR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01295)
Host
Rabbit
Contents
FLIP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FLIP antibody was raised against a 17 amino acid peptide near the carboxy terminus of mouse FLIP. The immunogen is located within the last 50 amino acids of FLIP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
FLIP has short form (FLIPS) and long form (FLIPL) and antibody recognizes the FLIPL only.
Reactive Species
A01295 is reactive to CFLAR in Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
55344 MW
Background of FLIP
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family.Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and MRIT. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01295 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FLIP antibody can be used for the detection of FLIP by Western blot at 1-2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2 μg/ml.
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Immunocytochemistry of FLIP in 3T3 cells with FLIP antibody at 5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For CFLAR (Source: Uniprot.org, NCBI)
Gene Name
CFLAR
Full Name
CASP8 and FADD-like apoptosis regulator
Weight
55344 MW
Superfamily
peptidase C14A family
Alternative Names
CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, c-FLIPL, c-FLIPR, c-FLIPS, CASP8AP1, CASH, CASP8 and FADD-like apoptosis regulator, Caspase homolog CFLAR CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS, cFLIP CASP8 and FADD like apoptosis regulator CASP8 and FADD-like apoptosis regulator|FADD-like antiapoptotic molecule 1|MACH-related inducer of toxicity|caspase homolog|caspase-eight-related protein|caspase-like apoptosis regulatory protein|caspase-related inducer of apoptosis|cellular FLICE-like inhibitory protein|inhibitor of FLICE|testis secretory sperm-binding protein Li 233m|usurpin beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CFLAR, check out the CFLAR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CFLAR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FLIP CFLAR Antibody (A01295)
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