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Product Info Summary
| SKU: | A02859-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Talin 1/TLN1 Antibody Picoband®
SKU/Catalog Number
A02859-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Talin 1/TLN1 Antibody Picoband® catalog # A02859-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Talin 1/TLN1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02859-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Talin 1, which shares 97.6% amino acid (aa) sequence identity with both mouse and rat Talin 1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02859-1 is reactive to TLN1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
270 kDa
Calculated molecular weight
36043 MW
Background of Talin1
Talin-1 is a protein that in humans is encoded by the TLN1 gene. This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02859-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human Jurkat whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell
IHC: human Lung cancer tissue, mouse small intestine tissue, human intestinal cancer tissue, human tonsil tissue, rat small intestine tissue, rat lung tissue, rat spleen tissue, mouse spleen tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Talin 1/TLN1 using anti-Talin 1/TLN1 antibody (A02859-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Talin 1/TLN1 antigen affinity purified polyclonal antibody (Catalog # A02859-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Talin 1/TLN1 at approximately 270 kDa. The expected band size for Talin 1/TLN1 is at 270 kDa.
Click image to see more details
Figure 2. IHC analysis of Talin 1 using anti-Talin 1 antibody (A02859-1).
Talin 1 was detected in paraffin-embedded section of human Lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Talin 1 Antibody (A02859-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Talin 1 using anti-Talin 1 antibody (A02859-1).
Talin 1 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Talin 1 Antibody (A02859-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Talin 1 using anti-Talin 1 antibody (A02859-1).
Talin 1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Talin 1 Antibody (A02859-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Talin 1 using anti-Talin 1 antibody (A02859-1).
Talin 1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Talin 1 Antibody (A02859-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of Talin 1 using anti-Talin 1 antibody (A02859-1).
Talin 1 was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Talin 1 Antibody (A02859-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of Talin 1 using anti-Talin 1 antibody (A02859-1).
Talin 1 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Talin 1 Antibody (A02859-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 8. IHC analysis of Talin 1 using anti-Talin 1 antibody (A02859-1).
Talin 1 was detected in paraffin-embedded section of rat lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Talin 1 Antibody (A02859-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 9. IHC analysis of Talin 1 using anti-Talin 1 antibody (A02859-1).
Talin 1 was detected in paraffin-embedded section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Talin 1 Antibody (A02859-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For TLN1 (Source: Uniprot.org, NCBI)
Gene Name
TLN1
Full Name
Talin-1
Weight
36043 MW
Alternative Names
Talin-1; TLN1; KIAA1027; TLN TLN1 ILWEQ, TLN, talin-1 talin 1 talin-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TLN1, check out the TLN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TLN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Talin 1/TLN1 Antibody Picoband® (A02859-1)
Hello CJ!
A02859-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Talin promotes integrin activation accompanied by generation of tension in talin and an increase in osmotic pressure in neurite outgrowth
Song Y,Li C,Fu Y,Xie Q,Guo J,Li G,Wu H.Inward Tension of Talin and Integrin-related Osmotic Pressure are involved Synergetically in the Invasion and Metastasis of Non-small Cell Lung Cancer.J Cancer.2020 Jun 23;11(17):5032-5041.doi:10.7150/jca.45494.PMID:
Species: Human
A02859-1 usage in article: APP:WB, SAMPLE:A549 CELL, DILUTION:1:500
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Customer Q&As
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7 Customer Q&As for Anti-Talin 1/TLN1 Antibody Picoband®
Question
Can you help my question with product A02859-1, anti-Talin 1/TLN1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02859-1 anti-Talin 1/TLN1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-24
Question
I was wanting to use your anti-Talin 1/TLN1 antibody for IHC for mouse embryonic carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse embryonic carcinoma identification?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
It shows on the product datasheet, A02859-1 anti-Talin 1/TLN1 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse embryonic carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-12
Question
We are currently using anti-Talin 1/TLN1 antibody A02859-1 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
The anti-Talin 1/TLN1 antibody (A02859-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-25
Question
See below the WB image, lot number and protocol we used for embryonic carcinoma using anti-Talin 1/TLN1 antibody A02859-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-30
Question
Is a blocking peptide available for product anti-Talin 1/TLN1 antibody (A02859-1)?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
We do provide the blocking peptide for product anti-Talin 1/TLN1 antibody (A02859-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-16
Question
Does anti-Talin 1/TLN1 antibody A02859-1 work for IHC with embryonic carcinoma?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
According to the expression profile of embryonic carcinoma, TLN1 is highly expressed in embryonic carcinoma. So, it is likely that anti-Talin 1/TLN1 antibody A02859-1 will work for IHC with embryonic carcinoma.
Boster Scientific Support
Answered: 2019-06-05
Question
I would like to test anti-Talin 1/TLN1 antibody A02859-1 on mouse embryonic carcinoma for research purposes, then I may be interested in using anti-Talin 1/TLN1 antibody A02859-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Singh
Verified customer
Asked: 2018-07-16
Answer
The products we sell, including anti-Talin 1/TLN1 antibody A02859-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-16
