Anti-FLIP (human) Monoclonal Antibody (7F10)

Product Name

Anti-FLIP (human) Monoclonal Antibody (7F10)

View all FLIP Antibodies

SKU/Catalog Number

M01295

Size

100µg

Form

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Description

Mouse Monoclonal 7F10 antibody for CFLAR detection. Tested positive for ELISA, Flow Cytometry, IHC, WB in Human

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FLIP (human) Monoclonal Antibody (7F10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01295)

Host

Mouse

Contents

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Clonality

Monoclonal

Clone Number

7F10

Isotype

IgG1, kappa

Immunogen

Recombinant human FLIP.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01295 is reactive to CFLAR in Human

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

10871 MW

Background of FLIP

The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2 and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, 2 and 3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include MDM2, p53, PML and RanGap1. SUMO-2 and 3 contribute to a greater percentage of protein modification than does SUMO-1 and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates beta-Amyloid generation and may be critical in the onset or progression of Alzheimer's disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01295 is guaranteed for ELISA, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1:500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

Gene/Protein Information For CFLAR (Source: Uniprot.org, NCBI)

Alternative Names

Small ubiquitin-related modifier 2 ;SUMO-2 ;HSMT3 ;SMT3 homolog 2 ;SUMO-3 ;Sentrin-2 ;Ubiquitin-like protein SMT3B ;Smt3B ;SUMO2 ;SMT3B , SMT3H2; CFLAR CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS, cFLIP CASP8 and FADD like apoptosis regulator CASP8 and FADD-like apoptosis regulator|FADD-like antiapoptotic molecule 1|MACH-related inducer of toxicity|caspase homolog|caspase-eight-related protein|caspase-like apoptosis regulatory protein|caspase-related inducer of apoptosis|cellular FLICE-like inhibitory protein|inhibitor of FLICE|testis secretory sperm-binding protein Li 233m|usurpin beta

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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