CFLAR Antibodies

CASP8 and FADD-like apoptosis regulator (CFLAR)

Apoptosis regulator protein which might function as a critical link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis.

A proteolytic fragment (p43) is probably retained in the death-inducing signaling complex (DISC) thereby blocking additional processing and recruiting of caspase-8 in the complex. Total length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis.

Gene Information

Human
Gene Name:	CFLAR
Uniprot:	O15519
Entrez:	8837

Family

Belongs to:
peptidase C14A family

Alternative Names

CASHFADD-like antiapoptotic molecule 1; CASP8 and FADD-like apoptosis regulator; CASP8AP1; Caspase homolog; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; caspase-related inducer of apoptosis; CASPER; CasperFLAME1; Cellular FLICE-like inhibitory protein; CFLAR; c-FLIPR; c-FLIPS; CLARPMACH-related inducer of toxicity; FADD-like apoptotic molecule; FLAME; FLAME-1; FLIP; I-FLICE; I-FLICEMRITc-FLIPFLIPc-FLIPL; Inhibitor of FLICE; usurpin beta; Usurpin

Molecular Weight

Mass (kDA):
55.344 kDA

Genomic Context

Human
Location:	2q33.1
Sequence:	2; NC_000002.12 (201116104..201176687)

Tissue Specificity

Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.

Diseases

• Malignant Neoplasms
• Neoplasms
• Carcinoma
• Lymphoma
• Leukemia
• Malignant Paraganglionic Neoplasm
• Inflammation
• Autoimmune Reaction
• Cell Transformation, Neoplastic
• Prostatic Neoplasms
• Melanoma
• Adenocarcinoma
• Malignant Neoplasm Of Prostate

• Autoimmune Diseases
• Malignant Tumor Of Colon
• Lung Neoplasms
• Colonic Neoplasms
• Rheumatoid Arthritis
• Tumor Progression
• Liver Carcinoma

Interacting Proteins

• CASP10
• CASP8
• MAP2K1
• MAP2K7
• TRAF1

Pathways

• Cell Death
• Sensitization
• Pathogenesis
• Induction Of Apoptosis
• Cell Proliferation
• Programmed Cell Death
• Cell Cycle
• Cell Growth
• Rna Interference
• Immune Response
• Cell Activation
• Localization
• Translation

• Secretion
• T Cell Activation
• Fas Signaling Pathway
• Angiogenesis
• Cell Differentiation
• Cell Cycle Arrest
• Methylation

PTMs

• Cleavage
• Phosphorylation
• Ubiquitination
• Methylation
• Acetylation
• Dephosphorylation

• Demethylation
• Reduction
• Nitrosylation
• Ribosylation
• Deacetylation
• Oxidation

• Palmitoylation

Research Areas

• Apoptosis
• Cancer
• Cell Biology
• Signal Transduction
• Tumor Suppressors

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CFLAR

What Are The Best Uses Of The CFLAR Marker?

The CFLAR Marker is a highly specific mAb used for labeling cells. It is widely used in cell biology and biotechnology. Its validation on WB and IHC as well as ICC will be discussed in this article. We will also discuss the process of validation for CFLAR Marker antibody. This information is beneficial for all applications that require labeling of cells. Continue reading to learn more. Also read What Are The Best Benefits Of The CFLAR Marker?

CFLAR Marker mAbs can be used to label cells

CFLAR marker mAbs that are used for labeling cells can be agonistic or antagonistic. They were created to label cells. An agonistic antibody blocks the FcgIII or FcgII receptors at the cell surface. The antibody is specifically targeted for cell surface markers, such as the CFLAR receptor on B cells. Cell labeling was done using mAb Clone 2.2.4G2.

Validation of WB

Picokine ELISA Kits are powered by patent-pending blocking technologies and coated technologies. They are tested against biologically relevant matrices such as blood plasma and supernatants from cell cultures. They are also tested for intra- and inter-assay variability to ensure that the results are consistent from lot one to lot. Boster has validation images available. These tests check the reproducibility and accuracy of the Boster ELISA kit.

Boster Bio is an antibody manufacturer that is specialized in picogram-level ELISA kit and IHC-optimized, polyclonal antibodies. The extensive range of products offered by Boster Bio has been validated for IHC WB, IHC, and Flow applications. Boster Bio stands behind its products and services by the Boster Guarantee. Boster Bio offers a broad product line and is constantly expanding to meet the needs of scientists from all over the world.

IHC

CFLAR Also known as c-FLIP , is a protein encoded in the CFLAR gene. While CASP8 has many similarities to caspase-8, it does not have caspase activity. Some researchers believe that the cFLIP gene is responsible for the development of human cancer. Numerous transcript variants have been discovered in the CFLAR gene, and further evidence supports these conclusions. Boster Bio validates all its antigens using IHC/WB. This is the reason it has such confidence in its products.

The Anti-FLIP CFLAR Antibody could be used to detect IF, ELISA and WB. It reacts with Human, Mouse, and Rat cells. The Boster Bio Anti-FLIP CFLAR antibody can also be tested in flow cytometry, ELISA, and CFLAR assays. It is available in two - and five-fold dilutions.

ICC

CFLAR is an immunohistochemical test that identifies an individual subunit of the LC3B or LC3A genes. It has high sensitivity as well as specificity. It can detect a variety of signal transduction pathways including GABARAP. CFLAR can be used for many purposes that include imaging neuronal activity and gene transcription. Here are some examples of its use in ICC.

Immunofluorescence

The CFLAR gene is expressed by various cells that include tumor cells as well as lymphoid tissues. Boster Bio's CFLAR antibody provides a fast and reliable method of analyzing these cells. Boster's antibody is compatible with both mouse and rat cells , and works well for immunofluorescence. Cell Signaling Technologies and eBiosciences have the antibodies.

Silibinin is an CFLAR inhibitor, blocks phosphorylation and up-regulates mRNA transcription in hepatic cell. It enhances the expression of NRF2, both HO-1 and CAT, as well as GSH Px. Additionally, it blocks CYP2E1 and CYP4A expression. This inhibitory effect of CFLAR protein results in the reduction of oxidative stress which increases hepatic injury.