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Product Info Summary
| SKU: | M02097-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-FH Mouse Monoclonal Antibody [Clone ID: OTI1F10]
SKU/Catalog Number
M02097-1
Size
100 µl
Description
Boster Bio FH (Fumarate Hydratase) mouse monoclonal antibody, clone OTI1F10 (formerly 1F10). Catalog# M02097-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-FH Mouse Monoclonal Antibody [Clone ID: OTI1F10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02097-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1F10
Isotype
IgG1
Immunogen
Full length human recombinant protein of human FH (NP_000134) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02097-1 is reactive to FH in Dog, Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
50 kDa
Calculated molecular weight
54.637kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02097-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FH.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-FH monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-FH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either FH (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FH antibody (M02097-1)
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Anti-FH mouse monoclonal antibody (M02097-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FH.
Protein Target Info & Infographic
Gene/Protein Information For FH (Source: Uniprot.org, NCBI)
Gene Name
FH
Full Name
Fumarate hydratase, mitochondrial
Weight
54.637kDa
Superfamily
class-II fumarase/aspartase family
Alternative Names
EC 4.2.1.2; fumarase; fumarate hydratase; fumarate hydratase, mitochondrial; HLRCC; LRCC; MCL; MCUL1 FH FMRD, HLRCC, HsFH, LRCC, MCL, MCUL1 fumarate hydratase fumarate hydratase, mitochondrial|epididymis secretory sperm binding protein|fumarase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FH, check out the FH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FH Mouse Monoclonal Antibody [Clone ID: OTI1F10] (M02097-1)
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