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Product Info Summary
| SKU: | A08751 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC |
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Product info
Product Name
Anti-F4/80/Adgre1 Antibody
SKU/Catalog Number
A08751
Size
100 µg/vial
Form
Liquid
Description
Boster Bio Anti-F4/80/Adgre1 Antibody catalog # A08751. Tested in IF, IHC applications. This antibody reacts with Mouse, Rat.
Storage & Handling
At -20˚C for one year, at 4˚C for one month. Avoid repeated freezing and thawing.
Cite This Product
Anti-F4/80/Adgre1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08751)
Host
Rabbit
Contents
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse F4/80/Adgre1 recombinant protein (Position: G27-S230).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08751 is reactive to Adgre1 in Mouse, Rat
Reconstitution
Observed Molecular Weight
66 kDa
Calculated molecular weight
97.683kDa
Background of ADGRE1
Predicted to enable G protein-coupled receptor activity. Predicted to be involved in adenylate cyclase-activating G protein-coupled receptor signaling pathway. Predicted to act upstream of or within G protein-coupled receptor signaling pathway and adaptive immune response. Located in external side of plasma membrane. Is expressed in several structures, including cardiovascular system; central nervous system; genitourinary system; hemolymphoid system; and intestine. Orthologous to human ADGRE1 (adhesion G protein-coupled receptor E1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08751 is guaranteed for IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Mouse, Rat
Immunofluorescence, 5 µg/ml, Mouse, Rat
Positive Control
IHC: rat liver tissue, mouse liver tissue, mouse spleen tissue
IF: mouse liver tissue, mouse spleen tissue, rat liver tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of F4/80/Adgre1 using anti-F4/80/Adgre1 antibody (A08751).
F4/80/Adgre1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-F4/80/Adgre1 Antibody (A08751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of F4/80/Adgre1 using anti-F4/80/Adgre1 antibody (A08751).
F4/80/Adgre1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-F4/80/Adgre1 Antibody (A08751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of F4/80/Adgre1 using anti-F4/80/Adgre1 antibody (A08751).
F4/80/Adgre1 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-F4/80/Adgre1 Antibody (A08751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of F4/80/Adgre1 using anti-F4/80/Adgre1 antibody (A08751).
F4/80/Adgre1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-F4/80/Adgre1 Antibody (A08751) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. IF analysis of F4/80/Adgre1 using anti-F4/80/Adgre1 antibody (A08751).
F4/80/Adgre1 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-F4/80/Adgre1 Antibody (A08751) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 6. IF analysis of F4/80/Adgre1 using anti-F4/80/Adgre1 antibody (A08751).
F4/80/Adgre1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-F4/80/Adgre1 Antibody (A08751) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For Adgre1 (Source: Uniprot.org, NCBI)
Gene Name
Adgre1
Full Name
Adhesion G protein-coupled receptor E1
Weight
97.683kDa
Superfamily
G-protein coupled receptor 2 family
Alternative Names
Transcription factor Sp6; Krueppel-like factor 14; SP6; KLF14 ADGRE1 EMR1, TM7LN3 adhesion G protein-coupled receptor E1 adhesion G protein-coupled receptor E1|EGF-like module receptor 1|EGF-like module-containing mucin-like hormone receptor-like 1|EMR1 hormone receptor|egf-like module containing, mucin-like, hormone receptor-like 1|egf-like module containing, mucin-like, hormone receptor-like sequence 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Adgre1, check out the Adgre1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Adgre1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-F4/80/Adgre1 Antibody (A08751)
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