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Product Info Summary
| SKU: | A00057S118-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ER Alpha (Phospho-S118) ESR1 Antibody
View all ER alpha/NR3A1 Antibodies
SKU/Catalog Number
A00057S118-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ER Alpha (Phospho-S118) ESR1 Antibody catalog # A00057S118-2. Tested in WB,ICC/IF,IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ER Alpha (Phospho-S118) ESR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00057S118-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser118 of human Estrogen Receptor alpha.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00057S118-2 is reactive to ESR1 in Human
Reconstitution
Calculated molecular weight
66216 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00057S118-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of ESR1 using Anti-ER Alpha (Phospho-S118) ESR1 Antibody (A00057S118-2).
ICC staining phospho-Estrogen Receptor alpha(S118) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-ER Alpha (Phospho-S118) ESR1 Antibody A00057S118-2
Western blot analysis of Estrogen Receptor alpha(phospho S118) on MCF-7 cell lysates using anti-phospho-Estrogen Receptor alpha(S118) antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For ESR1 (Source: Uniprot.org, NCBI)
Gene Name
ESR1
Full Name
Estrogen receptor
Weight
66216 MW
Superfamily
nuclear hormone receptor family
Alternative Names
Estrogen receptor;ER;ER-alpha;Estradiol receptor;Nuclear receptor subfamily 3 group A member 1;ESR1;ESR, NR3A1; ESR1 ER, ESR, ESRA, ESTRR, Era, NR3A1 estrogen receptor 1 estrogen receptor|E2 receptor alpha|ER-alpha|estradiol receptor|estrogen nuclear receptor alpha|estrogen receptor alpha E1-E2-1-2|estrogen receptor alpha E1-N2-E2-1-2|nuclear receptor subfamily 3 group A member 1|oestrogen receptor alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ESR1, check out the ESR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ESR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ER Alpha (Phospho-S118) ESR1 Antibody (A00057S118-2)
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