Click image to see more details
Product Info Summary
| SKU: | A01438-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Eotaxin/Ccl11 Antibody
View all CCL11/Eotaxin Antibodies
SKU/Catalog Number
A01438-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Eotaxin/Ccl11 Antibody catalog # A01438-2. Tested in ELISA, IHC applications. This antibody reacts with Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Eotaxin/Ccl11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01438-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat Eotaxin/Ccl11 recombinant protein (Position: H24-P97).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01438-2 is reactive to Ccl11 in Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
51561 MW
Background of CCL11/Eotaxin
CCL11 (Chemokine (C-C motif) ligand 11), also known as Eotaxin, is a small cytokine belonging to the CC chemokine family. Eotaxin selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in allergic responses. The effects of Eotaxin are mediated by its binding to a G-protein-linked receptor known as a chemokine receptor. Chemokine receptors for which Eotaxin is a ligand include CCR2, CCR3 and CCR5. However, it has been found that eotaxin-1 (CCL11) has high degree selectivity for its receptor, such that they are inactive on neutrophils and monocytes, which do not express CCR3. The gene for human Eotaxin is encoded on three exons and is located on chromosome 17q12.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01438-2 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Rat
ELISA, 0.1-0.5 μg/ml, Rat
Positive Control
IHC: rat lymphaden tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of Eotaxin/Ccl11 using anti-Eotaxin/Ccl11 antibody (A01438-2).
Eotaxin/Ccl11 was detected in a paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Eotaxin/Ccl11 Antibody (A01438-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Ccl11 (Source: Uniprot.org, NCBI)
Gene Name
Ccl11
Full Name
Eotaxin
Weight
51561 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
Bestrophin-1; TU15B; Vitelliform macular dystrophy protein 2; BEST1; VMD2 CCL11 SCYA11 C-C motif chemokine ligand 11 eotaxin|chemokine (C-C motif) ligand 11|eosinophil chemotactic protein|eotaxin-1|small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ccl11, check out the Ccl11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ccl11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Eotaxin/Ccl11 Antibody (A01438-2)
Hello CJ!
No publications found for A01438-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Eotaxin/Ccl11 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Eotaxin/Ccl11 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
