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Product Info Summary
| SKU: | EK0329 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (citrate, EDTA). |
Product info
Product Name
Human Eotaxin / CCL11 ELISA Kit PicoKine®
View all CCL11/Eotaxin ELISA kits
SKU/Catalog Number
EK0329
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Eotaxin / CCL11 ELISA Kit PicoKine® (96 Tests). Quantitate Human CCL11 in cell culture supernatants, serum and plasma (citrate, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Eotaxin / CCL11 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0329)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: G24-P97
Sensitivity
<2 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0329 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0329 is reactive to CCL11 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (citrate, EDTA).
Application Guarantee
EK0329 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL11/Eotaxin
Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3 (CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin. Recombinant human eotaxin produced in insect cells induces a calcium flux response in normal human eosinophils, but not in neutrophils or monocytes. The human eotaxin gene is cloned and found to be 61.8 and 63.2% identical at the amino acid level to guinea pig and mouse eotaxin.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0329-CAP | Anti-Human CCL11 Pre-coated 96-well strip microplate | 1 |
| EK0329-ST | Human CCL11 Standard | 2 vials, 10 ng/tube |
| EK0329-DA | Human CCL11 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.030 | 0.055 | 0.091 | 0.167 | 0.301 | 0.568 | 1.134 | 1.877 |
Data Example Images
Click image to see more details
Human Eotaxin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CCL11/Eotaxin, For serum, dilution ratio is 1:1 and concentration is 40-770 pg/ml.
For plasma, dilution ratio is 1:1 and concentration is 25-478 pg/ml..
Some articles we found to cite concentrations of CCL11/Eotaxin in samples: 25645983, 11346206 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Eotaxin / CCL11 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 36 | 181 | 476 | 34 | 176 | 517 |
| Standard deviation | 1.8 | 13.39 | 37.6 | 1.93 | 15.84 | 41.87 |
| CV (%) | 5% | 7.4% | 7.9% | 5.7% | 9% | 8.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CCL11/Eotaxin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 36 | 31 | 34 | 34 | 33 | 1.78 | 5.3% |
| Sample 2 | 181 | 203 | 202 | 192 | 194 | 8.9 | 4.5% |
| Sample 3 | 476 | 507 | 504 | 491 | 494 | 12.25 | 2.4% |
Protein Target Info & Infographic
Gene/Protein Information For CCL11 (Source: Uniprot.Org, NCBI)
Gene Name
CCL11
Full Name
Eotaxin
Weight
10732 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
Eotaxin;C-C motif chemokine 11;Eosinophil chemotactic protein;Small-inducible cytokine A11;CCL11;SCYA11; CCL11 SCYA11 C-C motif chemokine ligand 11 eotaxin|chemokine (C-C motif) ligand 11|eosinophil chemotactic protein|eotaxin-1|small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CCL11, check out the CCL11 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCL11: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Eotaxin / CCL11 ELISA Kit PicoKine® (EK0329)
Hello CJ!
EK0329 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Comorbidity has no impact on eosinophil inflammation in the upper airways or on severity of the sinonasal disease in patients with nasal polyps
Increased Type 2 Innate Lymphoid Cells in Patients with Drug Reaction with Eosinophilia and Systemic Symptoms Syndrome
Ge Y,Cheng R,Sun S,Zhang S,Li L,Jiang J,Yang C,Xuan X,Chen J.Fangxiao Formula alleviates airway inflammation and remodeling in rats with asthma via suppression of transforming growth factor -β/Smad3 signaling pathway.Biomed Pharmacother.2019 Nov;119:109429.doi:10.1016/j.biopha.2019.109429.Epub 2019 Sep 7.PMID:31505422.
Species: Rat
EK0329 usage in article: APP:ELISA, SAMPLE:BRONCHOALVEOLARLAVAGE FLUID, DILUTION:NA
Li L, Yu Ch, Ying Hz, Yu Jm. J Ethnopharmacol. 2013 May 2;147(1):238-44. Doi: 10.1016/J.Jep.2013.03.012. Epub 2013 Mar 15. Antiviral Effects Of Modified Dingchuan Decoction Against Respiratory Syncytial Virus Infection In Vitro And In An Immunosup...
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human Eotaxin / CCL11 ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for CCL11 ELISA kit? I used your CCL11 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CCL11 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-12-08
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CCL11 ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of CCL11 that can be declared/interpreted as positive by the above standard is 2pg/ml.
Boster Scientific Support
Answered: 2019-12-08
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
C. Lewis
Verified customer
Asked: 2019-10-04
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-10-04
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-02-28
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-02-28
Question
Q: Can CCL11 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-09-15
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-09-15
Question
Q: is it okay to use heparin plasma as samples in Human CCL11 Picokine® ELISA Kit (Catalog # EK0329)?
E. Banerjee
Verified customer
Asked: 2017-04-09
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of CCL11 causing disruption of its protein structure. CCL11 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CCL11 ELISA, treating samples with a number of anticoagulants and decided that citrate or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-04-09
Question
Q: if the enzyme conjugated CCL11 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL11 antigen?
Verified Customer
Verified customer
Asked: 2016-01-04
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-01-04
Question
Q: how to analyze ELISA data? I have obtained CCL11 level in plasma.
Verified Customer
Verified customer
Asked: 2015-08-29
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2015-08-29
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
D. Anderson
Verified customer
Asked: 2014-03-20
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2014-03-20
