Anti-Human Bestrophin DyLight® 488 conjugated BEST1 Antibody

Product Name

Anti-Human Bestrophin DyLight® 488 conjugated BEST1 Antibody

View all Bestrophin 1 Antibodies

SKU/Catalog Number

A01434-Dyl488

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-Human Bestrophin DyLight® 488 conjugated BEST1 Antibody catalog # A01434-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human Bestrophin DyLight® 488 conjugated BEST1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01434-Dyl488)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Bestrophin, which shares 62.5% amino acid (aa) sequence identity with mouse Bestrophin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01434-Dyl488 is reactive to BEST1 in Human

Reconstitution

Observed Molecular Weight

39 kDa

Calculated molecular weight

67.684kDa

Background of Bestrophin 1

Bestrophin-1 (Best1) is a protein that, in humans, is encoded by the BEST1 gene. This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01434-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells

Validation Images & Assay Conditions

Gene/Protein Information For BEST1 (Source: Uniprot.org, NCBI)

Alternative Names

Bestrophin-1; TU15B; Vitelliform macular dystrophy protein 2; BEST1; VMD2 BEST1 ARB, BEST, BMD, Best1V1Delta2, RP50, TU15B, VMD2 bestrophin 1 bestrophin-1|Best disease|vitelliform macular dystrophy protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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