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Product Info Summary
| SKU: | M00023 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF |
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Product info
Product Name
Anti-EGFR (Epidermal Growth Factor Receptor) Monoclonal Antibody
SKU/Catalog Number
M00023
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-EGFR (Epidermal Growth Factor Receptor) Monoclonal Antibody (Catalog # M00023). Tested in Flow Cytometry, IF applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-EGFR (Epidermal Growth Factor Receptor) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00023)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1mg/ml.
Clonality
Monoclonal
Clone Number
Clone: GFR450
Isotype
IgG2a, kappa
Immunogen
Recombinant extracellular domain of human EGFR protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00023 is reactive to EGFR in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
134277 MW
Background of EGFR
This monoclonal antibody recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00023 is guaranteed for Flow Cytometry, IF Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Flow Cytometry of human EGFR on A431 cells. Black: cells alone; Grey: Isotype control; Green: AF488-labeled Anti-EGFR Mouse Monoclonal Antibody (GFR450).
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SDS-PAGE analysis purified Anti-EGFR Mouse Monoclonal Antibody (GFR/450). Confirmation of integrity and purity of antibody.
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Confocal immunofluorescent analysis of A431 cells using AF488-labeled Anti-EGFR Monoclonal Antibody (GFR450) (green). DAPI was used to stain the cell nuclei (blue).
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Confocal immunofluorescent analysis of A431 cells using AF488-labeled Isotype Control MAb (IgG2a) (green). DAPI was used to stain the cell nuclei (blue). (Negative Control)
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Analysis of Protein Array containing more than 19,000 full-length human proteins using Anti-EGFR Mouse Monoclonal Antibody (GFR/450).
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt™ array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt™ are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Protein Target Info & Infographic
Gene/Protein Information For EGFR (Source: Uniprot.org, NCBI)
Gene Name
EGFR
Full Name
Epidermal growth factor receptor
Weight
134277 MW
Superfamily
protein kinase superfamily
Alternative Names
Epidermal growth factor receptor;2.7.10.1;Proto-oncogene c-ErbB-1;Receptor tyrosine-protein kinase erbB-1;EGFR;ERBB, ERBB1, HER1; EGFR ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA epidermal growth factor receptor epidermal growth factor receptor|avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor tyrosine kinase domain|erb-b2 receptor tyrosine kinase 1|proto-oncogene c-ErbB-1|receptor tyrosine-protein kinase erbB-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EGFR, check out the EGFR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EGFR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EGFR (Epidermal Growth Factor Receptor) Monoclonal Antibody (M00023)
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