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Product Info Summary
| SKU: | PB9388 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Syndecan 4/SDC4 Antibody Picoband®
View all Syndecan-4 Antibodies
SKU/Catalog Number
PB9388
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Syndecan 4/SDC4 Antibody Picoband® catalog # PB9388. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Syndecan 4/SDC4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9388)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Syndecan 4, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9388 is reactive to SDC4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
21642 MW
Background of Syndecan-4
Syndecan 4 is also known as SDC4. The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9388 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: MCF-7 Whole Cell, SW620 Whole Cell, Rat Lung Tissue
IHC: Human Mammary Cancer Tissue, Rat Intestine Tissue, Mouse Intestine Tissue
Validation Images & Assay Conditions
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Anti-Syndecan 4 Picoband antibody, PB9388, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Anti-Syndecan 4 Picoband antibody, PB9388, Western blotting
All lanes: Anti Syndecan 4 (PB9388) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: Rat Lung Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 22KD
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Anti-Syndecan 4 Picoband antibody, PB9388, IHC(P)
IHC(P): Rat Intestine Tissue
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Anti-Syndecan 4 Picoband antibody, PB9388, IHC(P)
IHC(P): Mouse Intestine Tissue
Protein Target Info & Infographic
Gene/Protein Information For SDC4 (Source: Uniprot.org, NCBI)
Gene Name
SDC4
Full Name
Syndecan-4
Weight
21642 MW
Superfamily
syndecan proteoglycan family
Alternative Names
Syndecan-4;SYND4;Amphiglycan;Ryudocan core protein;SDC4; SDC4 SYND4 syndecan 4 syndecan-4|amphiglycan|ryudocan amphiglycan|ryudocan core protein|syndecan 4 (amphiglycan, ryudocan)|syndecan proteoglycan 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SDC4, check out the SDC4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SDC4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Syndecan 4/SDC4 Antibody Picoband® (PB9388)
Hello CJ!
PB9388 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cai P,Lu Z,Jiang T,Wang Z,Yang Y,Zheng L,Zhao J.Syndecan-4 involves in the pathogenesis of rheumatoid arthritis by regulating the inflammatory response and apoptosis of fibroblast-like synoviocytes.J Cell Phys iol.2020 Feb;235(2):1746-1758.doi:10.1002/jcp
Species: Human
PB9388 usage in article: APP:WB, SAMPLE:FLSS, DILUTION:NA
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Customer Q&As
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5 Customer Q&As for Anti-Syndecan 4/SDC4 Antibody Picoband®
Question
I see that the anti-Syndecan 4/SDC4 antibody PB9388 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-10
Question
Would anti-Syndecan 4/SDC4 antibody PB9388 work for IHC with placenta?
Verified Customer
Verified customer
Asked: 2017-07-24
Answer
According to the expression profile of placenta, SDC4 is highly expressed in placenta. So, it is likely that anti-Syndecan 4/SDC4 antibody PB9388 will work for IHC with placenta.
Boster Scientific Support
Answered: 2017-07-24
Question
Here is the WB image, lot number and protocol we used for placenta using anti-Syndecan 4/SDC4 antibody PB9388. Please let me know if you require anything else.
F. Thomas
Verified customer
Asked: 2016-01-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-01-22
Question
My question regarding product PB9388, anti-Syndecan 4/SDC4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Kulkarni
Verified customer
Asked: 2015-01-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9388 anti-Syndecan 4/SDC4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-01-28
Question
We are currently using anti-Syndecan 4/SDC4 antibody PB9388 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
C. Taylor
Verified customer
Asked: 2014-06-27
Answer
The anti-Syndecan 4/SDC4 antibody (PB9388) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-06-27
