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Product Info Summary
| SKU: | EK1356 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates and serum. |
Product info
Product Name
Human Syndecan-4/SDC4 ELISA Kit PicoKine®
View all Syndecan-4 ELISA kits
SKU/Catalog Number
EK1356
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Syndecan-4/SDC4 ELISA Kit PicoKine® (96 Tests). Quantitate Human SDC4 in cell culture supernatants, cell lysates and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Syndecan-4/SDC4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1356)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E19-E145
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1356 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1356 is reactive to SDC4 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates and serum.
Application Guarantee
EK1356 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Syndecan-4
Syndecan-4 (SDC4) is a protein that in humans is encoded by the SDC4 gene. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. It is mapped to 20q13.12. Syndecan-4 is one of the four vertebrate syndecans and has a molecular weight of ~20 kDa. It is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. Syndecan-4 also regulates the actin cytoskeleton, cell adhesion, and cell migration. In addition, Syndecan-4 can activate protein kinase C (PKC). The variable domain of syndecan-4 could be a site of self-association. What’s more, Syndecan-4 also binds to phosphatidylinositol (4,5)-bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1356-CAP | Anti-Human SDC4 Pre-coated 96-well strip microplate | 1 |
| EK1356-ST | Human SDC4 Standard | 2 vials, 10 ng/tube |
| EK1356-DA | Human SDC4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.042 | 0.077 | 0.159 | 0.261 | 0.404 | 0.800 | 1.503 | 1.872 |
Data Example Images
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Human Syndecan-4/SDC4 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Syndecan-4, Dilution ratio of 1:1, concentration in serum is around 600 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Syndecan-4/SDC4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 94 | 673 | 2108 | 102 | 680 | 1958 |
| Standard deviation | 3.85 | 52.49 | 128.58 | 5.4 | 56.44 | 154.68 |
| CV (%) | 4.1% | 7.8% | 6.1% | 5.3% | 8.3% | 7.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Syndecan-4 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 94 | 82 | 81 | 78 | 83 | 6.09 | 7.3% |
| Sample 2 | 673 | 735 | 621 | 630 | 664 | 45.06 | 6.7% |
| Sample 3 | 2108 | 2092 | 1869 | 1903 | 1993 | 107.82 | 5.4% |
Protein Target Info & Infographic
Gene/Protein Information For SDC4 (Source: Uniprot.Org, NCBI)
Gene Name
SDC4
Full Name
Syndecan-4
Weight
21642 MW
Superfamily
syndecan proteoglycan family
Alternative Names
Syndecan-4;SYND4;Amphiglycan;Ryudocan core protein;SDC4; SDC4 SYND4 syndecan 4 syndecan-4|amphiglycan|ryudocan amphiglycan|ryudocan core protein|syndecan 4 (amphiglycan, ryudocan)|syndecan proteoglycan 4
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SDC4, check out the SDC4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SDC4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Syndecan-4/SDC4 ELISA Kit PicoKine® (EK1356)
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Customer Q&As
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5 Customer Q&As for Human Syndecan-4/SDC4 ELISA Kit PicoKine®
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Syndecan-4 using the EK1356 Human Syndecan-4 Picokine® ELISA Kit?
E. Kaur
Verified customer
Asked: 2019-11-17
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-11-17
Question
Q: can I use citrate plasma as samples in Human Syndecan-4 Picokine® ELISA Kit (Catalog # EK1356)?
W. Thompson
Verified customer
Asked: 2019-10-28
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Syndecan-4 causing disruption of its protein structure. Syndecan-4 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Syndecan-4 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-10-28
Question
Q: which procedure should I follow in order to thaw whole blood sample for Syndecan-4 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-11-14
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Syndecan-4 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-11-14
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-09-30
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-09-30
Question
Q: can you suggest the dilution ratio of serum samples for detection of Syndecan-4 in Human plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
L. Dhawan
Verified customer
Asked: 2018-09-23
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Syndecan-4 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-09-23
