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Product Info Summary
| SKU: | M02133 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-EAAT1 SLC1A3 Rabbit Monoclonal Antibody
View all EAAT1/GLAST-1/SLC1A3 Antibodies
SKU/Catalog Number
M02133
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-EAAT1 SLC1A3 Rabbit Monoclonal Antibody catalog # M02133. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EAAT1 SLC1A3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02133)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABFO-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human EAAT1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02133 is reactive to SLC1A3 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
32 kDa
Calculated molecular weight
59572 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02133 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
IHC: rat cerebellum tissue, human brain tissue
Validation Images & Assay Conditions
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Western blot analysis of EAAT1 expression in Mouse brain lysate.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-EAAT1 antibody at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-EAAT1 antibody.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/50) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Protein Target Info & Infographic
Gene/Protein Information For SLC1A3 (Source: Uniprot.org, NCBI)
Gene Name
SLC1A3
Full Name
Excitatory amino acid transporter 1
Weight
59572 MW
Superfamily
dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family
Alternative Names
Excitatory amino acid transporter 1;Sodium-dependent glutamate/aspartate transporter 1;GLAST-1;Solute carrier family 1 member 3;SLC1A3;EAAT1, GLAST, GLAST1; SLC1A3 EA6, EAAT1, GLAST, GLAST1 solute carrier family 1 member 3 excitatory amino acid transporter 1|GLAST-1|sodium-dependent glutamate/aspartate transporter 1|solute carrier family 1 (glial high affinity glutamate transporter), member 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC1A3, check out the SLC1A3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC1A3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EAAT1 SLC1A3 Rabbit Monoclonal Antibody (M02133)
Hello CJ!
M02133 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ding Y, Zhang K, Liu S, Zhang Q, Ma C, Bruce Ic, Zhang X. Exp Ther Med. 2014 Dec;8(6):1909-1913. Epub 2014 Oct 15. Tumor Necrosis Factor-?? Promotes The Expression Of Excitatory Amino-Acid Transporter 2 In Astrocytes: Optimal Concentration And Inc...
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Customer Q&As
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6 Customer Q&As for Anti-EAAT1 SLC1A3 Rabbit Monoclonal Antibody
Question
Does this antibody bind to intra- or extra-cellular domains of EAAT1?
Verified customer
Asked: 2022-07-19
Answer
Thank you for your question. Below is the Information for you. M02133 CFKQFKTNYEKRSFKVP extra-cellular
Boster Scientific Support
Answered: 2022-07-19
Question
What is the sequence of the peptide that was used as immunogen? If it is proprietary, could you provide a range or general location? The customer is hoping to check whether the epitope of this antibody is in the extracellular domain of the protein- do you know?
Verified customer
Asked: 2022-04-21
Answer
The immunogen sequence for M02133 is CFKQFKTNYEKRSFKVP. The sequence is in the extracellular domain. https://www.uniprot.org/uniprot/P43003#sequences
Boster Scientific Support
Answered: 2022-04-21
Question
See attached the WB image, lot number and protocol we used for brain using anti-EAAT1 Rabbit Monoclonal antibody M02133. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-22
Question
Does anti-EAAT1 Rabbit Monoclonal antibody M02133 work on canine WB with brain cortex?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
Our lab technicians have not tested anti-EAAT1 Rabbit Monoclonal antibody M02133 on canine. You can run a BLAST between canine and the immunogen sequence of anti-EAAT1 Rabbit Monoclonal antibody M02133 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain cortex in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-16
Question
I was wanting to use your anti-EAAT1 Rabbit Monoclonal antibody for WB for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
R. Anderson
Verified customer
Asked: 2017-11-02
Answer
As indicated on the product datasheet, M02133 anti-EAAT1 Rabbit Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-02
Question
Does M02133 anti-EAAT1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Williams
Verified customer
Asked: 2015-12-07
Answer
As indicated on the product datasheet, M02133 anti-EAAT1 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-12-07
