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WB: Human Placenta Tissue Lysate
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WB: Human Placenta Tissue Lysate
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WB: Human Placenta Tissue Lysate
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Key=protein_name, value=Excitatory amino acid transporter 3
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Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
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Key=isotype, value=Rabbit IgG
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Key=sample_size_available, value=30ug for $99, contact us for details
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Key=cj_related_products, value=Aspartate Transport:PA2164-2^Glycine Transport:PA2164-2^L-glutamate Transport:PA2164-2
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Key=description, value=Boster Bio Anti-EAAT3/SLC1A1 Antibody catalog # PA2164-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-EAAT3/SLC1A1 Antibody catalog # PA2164-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-EAAT3/SLC1A1 Antibody
Key=contents, value=Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Key=background, value=Solute carrier family 1 member 1, also called SLC1A1, is a protein that in humans is encoded by the SLC1A1 gene. By Southern analysis of a panel of human/rodent somatic cell hybrids and by fluorescence in situ hybridization (FISH), this gene is mapped to 9p24.2. This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are though to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect.
Key=gene_name, value=SLC1A1
Key=synonyms, value=Excitatory amino acid transporter 3;Excitatory amino-acid carrier 1;Neuronal and epithelial glutamate transporter;Sodium-dependent glutamate/aspartate transporter 3;Solute carrier family 1 member 1;SLC1A1;EAAC1, EAAT3;
Key=application_details, value=Western blot, 0.1-0.5μg/ml, Human

Key=subcellular_localization, value= Cell membrane ; Multi-pass membrane protein. Apical cell membrane ; Multi-pass membrane protein. Membrane; Multi-pass membrane protein.
Key=tissue_specificity, value= Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers). .
Key=protein_function, value= Transports L-glutamate, L- and D-aspartate and L-cystein (PubMed:21123949). Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5 (By similarity). .
Key=application_notes, value=Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 25658|sc 7761
Key=immunogen, value=A synthetic peptide corresponding to a sequence at the N-terminus of human EAAT3.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
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Anti-EAAT3/SLC1A1 Antibody Picoband®

EAAT3 antibody

Boster Bio Anti-EAAT3/SLC1A1 Antibody catalog # PA2164-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PA2164-1
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-EAAT3/SLC1A1 Antibody Picoband®

View all EAAT3 Antibodies

SKU/Catalog Number

PA2164-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-EAAT3/SLC1A1 Antibody catalog # PA2164-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EAAT3/SLC1A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2164-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human EAAT3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2164-1 is reactive to SLC1A1 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

15 kDa

Calculated molecular weight

57100 MW

Background of EAAT3

Solute carrier family 1 member 1, also called SLC1A1, is a protein that in humans is encoded by the SLC1A1 gene. By Southern analysis of a panel of human/rodent somatic cell hybrids and by fluorescence in situ hybridization (FISH), this gene is mapped to 9p24.2. This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are though to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA2164-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: Human Placenta Tissue

Validation Images & Assay Conditions

Gene/Protein Information For SLC1A1 (Source: Uniprot.org, NCBI)

Gene Name

SLC1A1

Full Name

Excitatory amino acid transporter 3

Weight

57100 MW

Superfamily

dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family

Alternative Names

Excitatory amino acid transporter 3;Excitatory amino-acid carrier 1;Neuronal and epithelial glutamate transporter;Sodium-dependent glutamate/aspartate transporter 3;Solute carrier family 1 member 1;SLC1A1;EAAC1, EAAT3; SLC1A1 DCBXA, EAAC1, EAAT3, SCZD18 solute carrier family 1 member 1 excitatory amino acid transporter 3|excitatory amino acid carrier 1|neuronal and epithelial glutamate transporter|sodium-dependent glutamate/aspartate transporter 3|solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SLC1A1, check out the SLC1A1 Infographic

SLC1A1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SLC1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
PA2164-1

100μg

$370
PA2164-1-10ug

10μg sample (liquid)

$99
PA2164-1-Biotin

100 μg Biotin conjugated

$570
PA2164-1-Cy3

100 μg Cy3 conjugated

$570
PA2164-1-Dylight488

100 μg Dylight488 conjugated

$570
PA2164-1-Dylight550

100 μg Dylight550 conjugated

$570
PA2164-1-Dylight594

100 μg Dylight594 conjugated

$570
PA2164-1-FITC

100 μg FITC conjugated

$570
PA2164-1-HRP

100 μg HRP conjugated

$570
PA2164-1-APC

100 μg APC conjugated

$670
PA2164-1-PE

100 μg PE conjugated

$670
PA2164-1-iFluor647

100 μg iFluor647 conjugated

$670
PA2164-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PA2164-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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