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Key=meta_description, value=Boster Bio Anti-EAAT3/SLC1A1 Antibody catalog # PA2164-2. Tested in WB applications. This antibody reacts with Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody.
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Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Heart Tissue Lysate
Lane 3: HEPA Cell Lysate
Key=small_image_label, value=Anti-EAAT3 antibody, PA2164-2, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Heart Tissue Lysate
Lane 3: HEPA Cell Lysate
Key=thumbnail_label, value=Anti-EAAT3 antibody, PA2164-2, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Heart Tissue Lysate
Lane 3: HEPA Cell Lysate
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Key=size, value=100 μg/vial
Key=protein_name, value=Excitatory amino acid transporter 3
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Key=uniprot_id, value=P51906
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Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Key=predicted_reactivity
Key=source_company, value=Boster PA2164-2
Key=isotype, value=Rabbit IgG
Key=molecular_weight, value=56694 MW
Key=sample_size_available, value=30ug for $99, contact us for details
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Key=product_category, value=Primary Antibodies
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Key=description, value=Boster Bio Anti-EAAT3/SLC1A1 Antibody catalog # PA2164-2. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-EAAT3/SLC1A1 Antibody catalog # PA2164-2. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-EAAT3/SLC1A1 Antibody
Key=contents, value=Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Key=background, value=Solute carrier family 1 member 1, also called SLC1A1, is a protein that in humans is encoded by the SLC1A1 gene. By Southern analysis of a panel of human/rodent somatic cell hybrids and by fluorescence in situ hybridization (FISH), this gene is mapped to 9p24.2. This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are though to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect.
Key=gene_name, value=Slc1a1
Key=synonyms, value=Excitatory amino acid transporter 3;Excitatory amino-acid carrier 1;Sodium-dependent glutamate/aspartate transporter 3;Solute carrier family 1 member 1;Slc1a1;Eaac1, Eaat3;
Key=application_details, value=Western blot, 0.1-0.5μg/ml, Mouse, Rat

Key=subcellular_localization, value= Cell membrane ; Multi-pass membrane protein. Apical cell membrane ; Multi-pass membrane protein. Membrane; Multi-pass membrane protein.
Key=tissue_specificity, value= Expressed in brain, lung, kidney and skeletal muscle.
Key=protein_function, value= Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium (By similarity). Negatively regulated by ARL6IP5 (PubMed:12119102). .
Key=application_notes, value=Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 25658|sc 7761
Key=immunogen, value=A synthetic peptide corresponding to a sequence at the N-terminus of mouse EAAT3, identical to the related rat sequence.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=15 kDa
Key=custom_attribute_2, value=WB: Rat Liver Tissue, Rat Heart Tissue, HEPA Cell
Key=custom_attribute_3, value=BA2252-2
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Anti-EAAT3/SLC1A1 Antibody Picoband®

EAAT3 antibody

Boster Bio Anti-EAAT3/SLC1A1 Antibody catalog # PA2164-2. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PA2164-2
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-EAAT3/SLC1A1 Antibody Picoband®

View all EAAT3 Antibodies

SKU/Catalog Number

PA2164-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-EAAT3/SLC1A1 Antibody catalog # PA2164-2. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EAAT3/SLC1A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2164-2)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse EAAT3, identical to the related rat sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2164-2 is reactive to Slc1a1 in Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

15 kDa

Calculated molecular weight

56694 MW

Background of EAAT3

Solute carrier family 1 member 1, also called SLC1A1, is a protein that in humans is encoded by the SLC1A1 gene. By Southern analysis of a panel of human/rodent somatic cell hybrids and by fluorescence in situ hybridization (FISH), this gene is mapped to 9p24.2. This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are though to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA2164-2 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat

Positive Control

WB: Rat Liver Tissue, Rat Heart Tissue, HEPA Cell

Validation Images & Assay Conditions

Gene/Protein Information For Slc1a1 (Source: Uniprot.org, NCBI)

Gene Name

Slc1a1

Full Name

Excitatory amino acid transporter 3

Weight

56694 MW

Superfamily

dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family

Alternative Names

Excitatory amino acid transporter 3;Excitatory amino-acid carrier 1;Sodium-dependent glutamate/aspartate transporter 3;Solute carrier family 1 member 1;Slc1a1;Eaac1, Eaat3; SLC1A1 DCBXA, EAAC1, EAAT3, SCZD18 solute carrier family 1 member 1 excitatory amino acid transporter 3|excitatory amino acid carrier 1|neuronal and epithelial glutamate transporter|sodium-dependent glutamate/aspartate transporter 3|solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Slc1a1, check out the Slc1a1 Infographic

Slc1a1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Slc1a1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-EAAT3/SLC1A1 Antibody Picoband®

Question

Will anti-EAAT3/SLC1A1 antibody PA2164-2 work on feline WB with brain cortex?

Verified Customer

Verified customer

Asked: 2019-05-23

Answer

Our lab technicians have not validated anti-EAAT3/SLC1A1 antibody PA2164-2 on feline. You can run a BLAST between feline and the immunogen sequence of anti-EAAT3/SLC1A1 antibody PA2164-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline brain cortex in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-23

Question

I am looking for to test anti-EAAT3/SLC1A1 antibody PA2164-2 on rat placenta for research purposes, then I may be interested in using anti-EAAT3/SLC1A1 antibody PA2164-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

N. Anderson

Verified customer

Asked: 2019-04-09

Answer

The products we sell, including anti-EAAT3/SLC1A1 antibody PA2164-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-04-09

Question

We are currently using anti-EAAT3/SLC1A1 antibody PA2164-2 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2018-10-22

Answer

The anti-EAAT3/SLC1A1 antibody (PA2164-2) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-22

Question

Does PA2164-2 anti-EAAT3/SLC1A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

L. Gonzalez

Verified customer

Asked: 2014-07-02

Answer

As indicated on the product datasheet, PA2164-2 anti-EAAT3/SLC1A1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-07-02

Order DetailsPrice
PA2164-2

100μg

$370
PA2164-2-10ug

10μg sample (liquid)

$99
PA2164-2-Biotin

100 μg Biotin conjugated

$570
PA2164-2-Cy3

100 μg Cy3 conjugated

$570
PA2164-2-Dylight488

100 μg Dylight488 conjugated

$570
PA2164-2-Dylight550

100 μg Dylight550 conjugated

$570
PA2164-2-Dylight594

100 μg Dylight594 conjugated

$570
PA2164-2-FITC

100 μg FITC conjugated

$570
PA2164-2-HRP

100 μg HRP conjugated

$570
PA2164-2-APC

100 μg APC conjugated

$670
PA2164-2-PE

100 μg PE conjugated

$670
PA2164-2-iFluor647

100 μg iFluor647 conjugated

$670
PA2164-2-carrier-free

Carrier Free

$370

More conjugates/formats

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PA2164-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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