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Product Info Summary
| SKU: | PA2164-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-EAAT3/SLC1A1 Antibody Picoband®
SKU/Catalog Number
PA2164-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EAAT3/SLC1A1 Antibody catalog # PA2164-2. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EAAT3/SLC1A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2164-2)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse EAAT3, identical to the related rat sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2164-2 is reactive to Slc1a1 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
15 kDa
Calculated molecular weight
56694 MW
Background of EAAT3
Solute carrier family 1 member 1, also called SLC1A1, is a protein that in humans is encoded by the SLC1A1 gene. By Southern analysis of a panel of human/rodent somatic cell hybrids and by fluorescence in situ hybridization (FISH), this gene is mapped to 9p24.2. This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are though to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2164-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: Rat Liver Tissue, Rat Heart Tissue, HEPA Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-EAAT3 antibody, PA2164-2, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Heart Tissue Lysate
Lane 3: HEPA Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For Slc1a1 (Source: Uniprot.org, NCBI)
Gene Name
Slc1a1
Full Name
Excitatory amino acid transporter 3
Weight
56694 MW
Superfamily
dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family
Alternative Names
Excitatory amino acid transporter 3;Excitatory amino-acid carrier 1;Sodium-dependent glutamate/aspartate transporter 3;Solute carrier family 1 member 1;Slc1a1;Eaac1, Eaat3; SLC1A1 DCBXA, EAAC1, EAAT3, SCZD18 solute carrier family 1 member 1 excitatory amino acid transporter 3|excitatory amino acid carrier 1|neuronal and epithelial glutamate transporter|sodium-dependent glutamate/aspartate transporter 3|solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Slc1a1, check out the Slc1a1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Slc1a1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EAAT3/SLC1A1 Antibody Picoband® (PA2164-2)
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No publications found for PA2164-2
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Customer Q&As
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4 Customer Q&As for Anti-EAAT3/SLC1A1 Antibody Picoband®
Question
Will anti-EAAT3/SLC1A1 antibody PA2164-2 work on feline WB with brain cortex?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
Our lab technicians have not validated anti-EAAT3/SLC1A1 antibody PA2164-2 on feline. You can run a BLAST between feline and the immunogen sequence of anti-EAAT3/SLC1A1 antibody PA2164-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline brain cortex in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-23
Question
I am looking for to test anti-EAAT3/SLC1A1 antibody PA2164-2 on rat placenta for research purposes, then I may be interested in using anti-EAAT3/SLC1A1 antibody PA2164-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Anderson
Verified customer
Asked: 2019-04-09
Answer
The products we sell, including anti-EAAT3/SLC1A1 antibody PA2164-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-09
Question
We are currently using anti-EAAT3/SLC1A1 antibody PA2164-2 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-22
Answer
The anti-EAAT3/SLC1A1 antibody (PA2164-2) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-22
Question
Does PA2164-2 anti-EAAT3/SLC1A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Gonzalez
Verified customer
Asked: 2014-07-02
Answer
As indicated on the product datasheet, PA2164-2 anti-EAAT3/SLC1A1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-07-02
