Click image to see more details
Product Info Summary
| SKU: | PA2185 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-EAAT1/SLC1A3 Antibody Picoband®
View all EAAT1/GLAST-1/SLC1A3 Antibodies
SKU/Catalog Number
PA2185
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EAAT1/SLC1A3 Antibody catalog # PA2185. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EAAT1/SLC1A3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2185)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human EAAT1, different from the related rat and mouse sequences by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2185 is reactive to SLC1A3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
274 kDa
Calculated molecular weight
59572 MW
Background of EAAT1/GLAST-1/SLC1A3
Solute carrier family 1 (glial high-affinity glutamate transporter), member 3, also known as SLC1A3, EAAT1 or GLAST, is a protein that in humans is encoded by the SLC1A3 gene. This gene is a member of high affinity glutamate transporter family. SLC1A3 is mapped to chromosome 5p13.2 by fluorescence in situ hybridization (FISH). This gene transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. This gene acts as a symport by cotransporting sodium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2185 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue
IHC: Rat Brain Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-EAAT1 antibody, PA2185, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Click image to see more details
Anti-EAAT1 antibody, PA2185, IHC(P)
IHC(P): Rat Brain Tissue
Protein Target Info & Infographic
Gene/Protein Information For SLC1A3 (Source: Uniprot.org, NCBI)
Gene Name
SLC1A3
Full Name
Excitatory amino acid transporter 1
Weight
59572 MW
Superfamily
dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family
Alternative Names
Excitatory amino acid transporter 1;Sodium-dependent glutamate/aspartate transporter 1;GLAST-1;Solute carrier family 1 member 3;SLC1A3;EAAT1, GLAST, GLAST1; SLC1A3 EA6, EAAT1, GLAST, GLAST1 solute carrier family 1 member 3 excitatory amino acid transporter 1|GLAST-1|sodium-dependent glutamate/aspartate transporter 1|solute carrier family 1 (glial high affinity glutamate transporter), member 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC1A3, check out the SLC1A3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC1A3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EAAT1/SLC1A3 Antibody Picoband® (PA2185)
Hello CJ!
PA2185 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Improved modeling of human AD with an automated culturing platform for iPSC neurons, astrocytes and microglia
Ho, S.M., Hartley, B.J., Flaherty, E., Rajarajan, P., Abdelaal, R., Obiorah, I.,..., & Brennand, K.J. (2017). Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes. Stem Cel...
Ding Y, Zhang K, Liu S, Zhang Q, Ma C, Bruce Ic, Zhang X. Exp Ther Med. 2014 Dec;8(6):1909-1913. Epub 2014 Oct 15. Tumor Necrosis Factor-?? Promotes The Expression Of Excitatory Amino-Acid Transporter 2 In Astrocytes: Optimal Concentration And Inc...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-EAAT1/SLC1A3 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-EAAT1/SLC1A3 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
