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Product Info Summary
| SKU: | PB9561 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-E Cadherin 1/CDH1 Antibody Picoband®
SKU/Catalog Number
PB9561
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-E Cadherin 1/CDH1 Antibody Picoband® catalog # PB9561. Tested in ELISA, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-E Cadherin 1/CDH1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9561)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human E Cadherin recombinant protein (Position: A286-A703). Human E Cadherin shares 79.7% and 80.9% amino acid (aa) sequence identity with mouse and rat E Cadherin, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9561 is reactive to CDH1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
130 kDa
Calculated molecular weight
97456 MW
Background of CDH1
CDH1 (Cadherin 1), also known as ECAD or UVO, is a protein that in humans is encoded by the CDH1 gene. Cadherin-1 is a classical member of the cadherin superfamily. By Southern analysis of DNA from a panel of mouse-human somatic cell hybrids, Mansouri et al. (1987, 1988) assigned the UVO gene to 16q (16p11-qter). Frebourg et al. (2006) found that in human embryos CDH1 is highly expressed at 4 and 5 weeks in the frontonasal prominence and at 6 weeks in the lateral and medial nasal prominences, and is therefore expressed during critical stages of lip and palate development. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9561 is guaranteed for ELISA, IF, IHC, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Mouse, Rat -
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5μg/ml, Human, Mouse
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human MCF-7 whole cell, human A431 whole cell
IHC: Human Placenta tissue, mouse intestine tissue, rat intestine tissue
IHC-F: Human Placenta tissue, mouse intestine tissue, rat intestine tissue, human placenta tissue
ICC/IF: MCF-7 cell
IF: human ileum tissue, human colon organoid tissue, mouse ileum tissue, mouse ileum organoid tissue, human colon cancer tissue, mouse colon tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of E Cadherin using anti-E Cadherin antibody (PB9561).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E Cadherin antigen affinity purified polyclonal antibody (Catalog # PB9561) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for E Cadherin at approximately 130 kDa. The expected band size for E Cadherin is at 97 kDa.
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Figure 2. IHC analysis of E Cadherin using anti-E Cadherin antibody (PB9561).
E Cadherin was detected in paraffin-embedded section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-E Cadherin Antibody (PB9561) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of E Cadherin using anti-E Cadherin antibody (PB9561).
E Cadherin was detected in frozen section of Human Placenta Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-E Cadherin Antibody (PB9561) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of E Cadherin using anti-E Cadherin antibody (PB9561).
E Cadherin was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-E Cadherin Antibody (PB9561) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of E Cadherin using anti-E Cadherin antibody (PB9561).
E Cadherin was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-E Cadherin Antibody (PB9561) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of E Cadherin using anti-E Cadherin antibody (PB9561).
E Cadherin was detected in frozen section of mouse intestine tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-E Cadherin Antibody (PB9561) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of E Cadherin using anti-E Cadherin antibody (PB9561).
E Cadherin was detected in frozen section of rat intestine tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-E Cadherin Antibody (PB9561) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 8. IHC analysis of E Cadherin using anti-E Cadherin antibody (PB9561).
E Cadherin was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-E Cadherin Antibody (PB9561) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 9. IF analysis of E Cadherin using anti-E Cadherin antibody (PB9561).
E Cadherin was detected in paraffin-embedded section of human ileum tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-E Cadherin Antibody (PB9561) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 10. IF analysis of E Cadherin using anti-E Cadherin antibody (PB9561).
E Cadherin was detected in paraffin-embedded section of human colon organoid tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-E Cadherin Antibody (PB9561) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 11. IF analysis of E Cadherin using anti-E Cadherin antibody (PB9561).
E Cadherin was detected in paraffin-embedded section of mouse ileum tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-E Cadherin Antibody (PB9561) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 12. IF analysis of E Cadherin using anti-E Cadherin antibody (PB9561).
E Cadherin was detected in paraffin-embedded section of mouse ileum organoid tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-E Cadherin Antibody (PB9561) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 13. IF analysis of E Cadherin using anti-E Cadherin antibody (PB9561).
E Cadherin was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-E Cadherin Antibody (PB9561) overnight at 4°C.DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 14. IF analysis of E Cadherin using anti-E Cadherin antibody (PB9561).
E Cadherin was detected in paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-E Cadherin Antibody (PB9561) overnight at 4°C.DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 15. IF analysis of E Cadherin using anti-E Cadherin antibody (PB9561).
E Cadherin was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-E Cadherin Antibody (PB9561) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CDH1 (Source: Uniprot.org, NCBI)
Gene Name
CDH1
Full Name
Cadherin-1
Weight
97456 MW
Alternative Names
Cadherin-1;CAM 120/80;Epithelial cadherin;E-cadherin;Uvomorulin;CD324;E-Cad/CTF1;E-Cad/CTF2;E-Cad/CTF3;CDH1;CDHE, UVO; CDH1 Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO cadherin 1 cadherin-1|CAM 120/80|E-cadherin 1|cadherin 1, E-cadherin (epithelial)|cadherin 1, type 1, E-cadherin (epithelial)|calcium-dependent adhesion protein, epithelial|cell-CAM 120/80|epididymis secretory sperm binding protein|epithelial cadherin|uvomorulin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDH1, check out the CDH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-E Cadherin 1/CDH1 Antibody Picoband® (PB9561)
Hello CJ!
PB9561 has been cited in 60 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Identification and differentiation therapy strategy of pterygium in vitro
Wnt/β-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells
RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells
Expression of RKIP, E-cadherin and NF-kB p65 in esophageal squamous cell carcinoma and their correlations
LINC00184 involved in the regulatory network of ANGPT2 via ceRNA mediated miR-145 inhibition in gastric cancer
Overexpression of SDF-1 activates the NF-κB pathway to induce epithelial to mesenchymal transition and cancer stem cell-like phenotypes of breast cancer cells
Microrchidia family CW‑type zinc finger 2 promotes the proliferation, invasion, migration and epithelial‑mesenchymal transition of glioma by regulating PTEN/PI3K/AKT signaling via binding to N‑myc downstream regulated gene 1 promoter
Effect of anaphylatoxin C3a, C5a on the tubular epithelial-myofibroblast transdifferentiationin vitro
Connective tissue growth factor is overexpressed in human hepatocellular carcinoma and promotes cell invasion and growth
Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial–mesenchymal transition via NF-κB activation
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Customer Reviews
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1 Reviews For Anti-E Cadherin 1/CDH1 Antibody Picoband®
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Immunocytochemistry for Anti-E Cadherin 1/CDH1 Antibody
Excellent
The antibody worked perfectly, Great sensitivity
| Application | Immunohistochemistry (paraffin-embedded) |
|---|---|
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Mouse Liver |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 4°C overnight |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 2ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | 4°C overnight |
E.Hernandez
Verified customer
Submitted 2020-07-18
Customer Q&As
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6 Customer Q&As for Anti-E Cadherin 1/CDH1 Antibody Picoband®
Question
Are there any optimizations that you suggest or Antigen retrieval for PB9561?
Verified customer
Asked: 2021-01-27
Answer
For the Anti-E Cadherin 1/CDH1 Antibody Picoband™ (PB9561), AR0022 is recommended to be used for Antigen retrieval. https://www.bosterbio.com/antigen-retrieval-reagent-ar0022-boster.html
Boster Scientific Support
Answered: 2021-01-28
Question
Our lab want to know about using your anti-E Cadherin/CDH1 antibody for regulation of gene expression studies. Has this antibody been tested with western blotting on placenta tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
We appreciate your inquiry. This PB9561 anti-E Cadherin/CDH1 antibody is tested on human ileum tissue, placenta tissue, tissue lysate, hela whole cell lysate, mouse ileum tissue, ileum organoid tissue, colon organoid tissue. It is guaranteed to work for ELISA, IF, IHC-P, IHC-F, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-20
Question
Our lab used your anti-E Cadherin/CDH1 antibody for IHC-F on hippocampus tongue in a previous experiment. I am using mouse, and I plan to use the antibody for IHC-P next. We want examining hippocampus tongue as well as pancreas in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
I viewed the website and datasheets of our anti-E Cadherin/CDH1 antibody and it appears that PB9561 has been tested on mouse in both IHC-F and IHC-P. Thus PB9561 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-12
Question
We are currently using anti-E Cadherin/CDH1 antibody PB9561 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
The anti-E Cadherin/CDH1 antibody (PB9561) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-05
Question
We have seen staining in rat liver. Do you have any suggestions? Is anti-E Cadherin/CDH1 antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
Based on literature liver does express CDH1. Based on Uniprot.org, CDH1 is expressed in jejunal mucosa, pancreas, liver, epidermal carcinoma, hippocampus tongue, placenta, brain, among other tissues. Regarding which tissues have CDH1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14595118
Epidermal carcinoma, Pubmed ID: 10597309
Hippocampus, and Tongue, Pubmed ID: 14702039
Liver, Pubmed ID: 3263290, 8185635, 24275569, 8033105, 11953314
Pancreas, Pubmed ID: 8459805
Placenta, Pubmed ID: 7543680, 7601454
Boster Scientific Support
Answered: 2019-06-17
Question
Our team were content with the WB result of your anti-E Cadherin/CDH1 antibody. However we have observed positive staining in epidermal carcinoma adherens junction using this antibody. Is that expected? Could you tell me where is CDH1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-12-17
Answer
From literature, epidermal carcinoma does express CDH1. Generally CDH1 expresses in cell junction, adherens junction. Regarding which tissues have CDH1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14595118
Epidermal carcinoma, Pubmed ID: 10597309
Hippocampus, and Tongue, Pubmed ID: 14702039
Liver, Pubmed ID: 3263290, 8185635, 24275569, 8033105, 11953314
Pancreas, Pubmed ID: 8459805
Placenta, Pubmed ID: 7543680, 7601454
Boster Scientific Support
Answered: 2018-12-17
